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Rev. Soc. Bras. Med. Trop ; 44(4): 528-530, July-Aug. 2011. ilus
Article in English | LILACS | ID: lil-596610

ABSTRACT

Flavivirus is a genus of arthropod-transmitted viruses of the family Flaviviridae, and in Brazil, up to eleven different Flavivirus have been isolated. We collected blood from farmers in the municipality of Theobroma, which is located 320km from the City of Porto Velho, the former capital of the Brazilian State of Rondônia. For viral isolation, we used newborn mouse brain, followed by RT-PCR with specific universal Flavivirus primers. We obtained fragments 958bp and 800bp in length. Based on BLAST, these sequences were 91% similar to a sequence of Cacipacore virus.


Flavivirus é um gênero dos vírus transmitidos por artrópode da família Flaviviridae e, no Brasil, são isolados onze Flavivirus diferentes. Foi coletado o sangue de um agricultor, no município de Theobroma situado a 320km de distância da Cidade de Porto Velho, capital do Estado Brasileiro, Rondônia. Para isolamento viral, foi usado cérebro de camundongos recém-nascido, seguido por RT-PCR com primers universais específicos de Flavivirus. Nós obtivemos fragmentos com 958bp e 800bp de comprimento. Ao Blast das sequências obtivemos 91% de similaridade com uma sequência do vírus de Cacipacoré.


Subject(s)
Animals , Humans , Male , Mice , Flavivirus Infections/virology , Flavivirus/genetics , Brazil/epidemiology , Flavivirus Infections/epidemiology , Flavivirus/classification , Flavivirus/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction , RNA, Viral/genetics
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