ABSTRACT
Background: Gold Nanoparticles [GNPs] are used in imaging and molecular diagnostic applications. As the development of a novel approach in the green synthesis of metal nanoparticles is of great importance and a necessity, a simple and safe method for the synthesis of GNPs using plant extracts of Zataria multiflora leaves was applied in this study and the results on GNPs' anticancer activity against HeLa cells were reported
Methods: The GNPs were characterized by UV-visible spectroscopy, FTIR, TEM, DLS and Zeta-potential measurements. In addition, the cellular up-take of nanoparticles was investigated using Dark Field Microscopy [DFM]. Induction of apoptosis by high dose of GNPs in HeLa cells was assessed by MTT assay, Acridin orange, DAPI staining, Annexin V/PI double-labeling flow cytometry and caspase activity assay
Results: UV-visible spectroscopy results showed a surface plasmon resonance band for GNPs at 530 nm. FTIR results demonstrated an interaction between plant extract and nanoparticles. TEM images revealed different shapes for GNPs and DLS results indicated that the GNPs range in size from 10 to 42 nm. The Zeta potential values of the synthesized GNPs were between 30 to 50 Mev, indicating the formation of stable particles. As evidenced by MTT assay, GNPs inhibit proliferation of HeLa cells in dosedependent GNPs and cytotoxicity of GNPs in Bone Marrow Mesenchymal Stem Cell [BMSCs] was lower than cancerous cells. At nontoxic concentrations, the cellular uptake of the nanoparticles took place. Acridin orange and DAPI staining showed morphological changes in the cell's nucleus due to apoptosis. Finally, caspase activity assay demonstrated HeLa cell's apoptosis through caspase activation
Conclusion: The results showed that GNPs have the ability to induce apoptosis in HeLa cells
ABSTRACT
Background and Aim: Stigmas of Crocus sativus L. is widely used against different human diseases. Regarding the properties of this plant, in the present study the effects of saffron extract on inducing cell differentiation of a rat's bone marrow-derived mesenchymal stem cells [MSCs] into osteoblasts was examined
Materials and Methods: Bone marrow cells collected from Bone of Wistar rat's femor and flowcytometry were used to identify them. In the experimental group [n=4] MSCs treatment was done with various concentrations of saffron extract [i.e. 0.5, 1, 1.5, and 2 mg/ml]. Cytotoxic effect of saffron extract was evaluated using MTT assay and its aqueous extract effect on cell differentiation was investigated by means of Alizarin staining and alkaline phosphates activity
Results: Flowcytometry results confirmed the presence of stem cells using CD44 antibody. MTT assay results showed that the extract concentration of 1.5 mg/ml resulted in the death of 50 percent of stem cells derived from the rats' bone marrow during 24 hours [P<0.001]. Alizarin staining showed saffron aqueous extract increased osteogenic s cell differentiation in a dose dependent manner in 21 days. [The maximum cell differentiation achieved by700 microg/ml concentration]. Besides, higher alkaline phosphates activity was evident in the experimental group compared to the control group [n=4] in the 14[th] day [P<0.001]
Conclusion: According to the findings of the current study, MSCs derived from the rat's bone marrow transform into osteoblasts when treated with saffron aqueous extract