ABSTRACT
The advances in the technology of in vitro fertilization [IVF] increases the incidence of success in treatment cases of infertile patients with non-obstructive azoospermia. Evaluation of the microscopic changes in the obtained testicular samples was one of the main aims in this study for better prognosis of the results. In this study one hundred human testicular biopsies were obtained from Islamic reproductive centre and Department of Urology Al-Hussein University Hospital. Cases in this study were classified into 4 groups: Group I includes 5 cases of normal fertile persons free of any medical problems and with normal seminal parameters. Patients consent was performed before sampling. Group II includes 35 cases of infertile obstructive azoospermic patients. All of the rest of cases [60] were considered infertile non-obstructive azoospermic cases and were included in both the third and the fourth groups. Group III: includes 20 cases of infertile non-obstructive positive patients. Group IV: includes 40 cases of infertile non-obstructive negative patients. Cases were either of normal sized testis or of hypoplastic testis and were subdivided into the following subgroups: I- Cases with normal sized testes [30] infertile patients: Group IV A: Normal sized testis with arrested spermatogenesis; Group IV B: Normal sized testis with SCOS; Group IVC: Normal sized testis with mixed atrophy. II- Cases with hypoplastic testes [10] infertile patients: Group IV D: Hypoplastic testis. The testicular samples were prepared for Histological and Histochemical examination. Different staining techniques were used: 1- Haematoxylin and Eosin stain; 2- Mallory's trichrome stain; and 3- Histochemical techniques: a- Periodic acid Schiff [PAS] technique. B- Methyl green pyronin. The obtained results showed no Morphological changes in group II except mild vascular dilation and congested peritubular capillaries. Cases of non-obstructive positive azoospermia showed reduced number of spermatogenic layers while cases of group IVa showed reduced size of the tubules and lined only by Sertoli cells and there was marked reduction in the number of leydig cells. In this study there were definite histochemical changes observed in both the PAS positive material and the nucleic acid in the different elements of the obtained testicular samples, Clinical and microscopic evaluations in this study could be of complementary importance and may increases of the incidence of success