ABSTRACT
By utilizing the antibody for rat DGKz a substantial number of immunopositive cells were found in the OV (Opisthorchis viverrini). The immunopositive cells appeared solitarily and they were distributed rather symmetrically to the longitudinal axis of the OV. Some of them were located in close proximity to internal organs such as uterus, ovary, testes, vitelline glands and guts. The immunostained cells extended tapering processes horizontally or obliquely to the OV longitudinal axis. In immuno-electron microscopy, the immunopositive cells were characterized by intensely immunostained mitochondria and weakly immunostained cytoplasm and immunonegative chromatin-poor nucleus. Vacuoles of various sizes without the immunoreactivity were also contained in the cells. Thin cellular processes without the immunoreactivity were found to enclose thinly the entire surfaces of the immunostained cells and processes, and they were in continuity with the interstitial partition-like processes which contained nuclei and aggregation of microfibrils at some distance from the cytoplasmic envelopes. The present finding suggests the possibility that the immunostained cells were peripheral neurons enveloped by peripheral glia and that the glia are of mesenchymal origin because of their cytoplasmic continuity to the interstitial partition-like processes. The motor or sensory nature of the neurons remains to be elucidated.
Mediante el uso del anticuerpos DGK para rata se determinó un número considerable de células inmunopositivas en el Opisthorchis viverrini (OV). Las células inmunopositivas aparecían solitarias y se distribuían simétricamente al eje longitudinal de la OV. Algunas estaban ubicadas en las proximidades de los órganos internos como el útero, ovarios, testículos, glándulas vitelinas e intestino. Las células inmunoteñidas extendían sus procesos horizontalmente u oblicuamente al eje longitudinal de la OV. Por microscopía inmunoelectrónica, las células inmunopositivas se caracterizaron por presentar mitocondrias intensamente teñidas, citoplasma con tinción débil e inmunonegatividad en núcleos pobres en cromatina. También se observó en las células, vacuolas de diversos tamaños sin inmunorreactividad. Se encontraron procesos celulares sin inmunorreactividad para cerrar finamente todas las superficies de las células y procesos, y se continuaron con los procesos de partición intersticiales que contenían núcleos y agregación de microfibrillas a cierta distancia de las envolturas citoplásmicas. El presente hallazgo sugiere la posibilidad de que las células inmunoteñidas son neuronas periféricas envueltas por glia periférica y que la glía presenta origen mesenquimal debido a su continuidad citoplasmática con los procesos de partición intersticiales. La naturaleza motora o sensorial de las neuronas aún no se ha dilucidado.
Subject(s)
Animals , Rats , Diacylglycerol Kinase/metabolism , Neurons/ultrastructure , Opisthorchis/ultrastructure , Peripheral Nerves/ultrastructure , Microscopy, Immunoelectron , Opisthorchis/immunologyABSTRACT
Objective: The aim of this study is to investigate the protective effect of Centella asiatica extract on gastric ulcers caused by indomethacin in rats. Methods: Male rats were orally given extract of C.asiatica prior to the administration of indomethacin. The stomach was dissected and the number of bleeding spots and histological features were determined. Results: Giving extract of C.asiatica at doses of 10 and 20 mg/kg for 30 minutes prior to oral administration of indomethacin decreased the number of bleeding spots are about 11 times lower than those given indomethacin alone. Histological findings of indomethacin-treated group showed the depth of injury of gastric mucosa which is about half the thickness of the mucosal layer. Treatment with C.asiatica decreased the depth of tissue erosion, down to one-third of the thickness of the mucosa. Conclusion: The results showed protective effect of C.asiatica on gastric ulceration caused by the anti-inflammatory drug, indomethacin.