ABSTRACT
Objective To analyze the potential association between the single ucleotide polymorphisms(SNP) of the vascular endothelial growth factor(VEGF) gene with the risk of endometriosis by meta-analysis.Methods Published case-control studies about the influence of VEGF polymorphisms on endometriosis were searched and screened in Medline,the Cochrane library,China National Knowledge Internet(CNKI),Chinese Biological Medicine Disk ( CBM ),data base of Wanfang and Foreign Medical Journal Full-Text Serv ice(FMJS).RevMan 5.0 software was used for meta-analysis.Results Finally,there were 9 literatures including 1610 endometrisis patients and 1643 controls cases,which were eligible for the criteria to investigating the VEGF SNP about - 460C/T,+ 405C/G and + 936C/T.The results of metaanalysis showed that there was no evidence for association between endometriosis and the VEGF -460C/T SNP in the genotype or allele frequencies distribution ( P ＞ 0.05 ).Significant differences were found between the frequencies distribution of VEGF + 405CC genotype( P =0.009,OR =1.45,95％ CI:1.10 - 1.91 ) and + 405 C allele ( P =0.020,OR =1.19,95 ％ CI:1.03 - 1.38 ),also between the + 936CC genotype ( P =0.050,OR=0.81,95％CI:0.66-1.00) and +936T allele (P=0.040,OR=1.20,95％CI:1.01 -1.43).Conclusions The VEGF + 405C/G and + 936C/T SNP may be associated with the risk of endometriosis.Women carrying the + 405C or the + 936T allele could significantly increase the risk of developing endometriosis.
ABSTRACT
<p><b>OBJECTIVE</b>To explore new toxicity-reducing methods of Pinellia Rhizoma prepared by ethanol and the latest technical parameters.</p><p><b>METHOD</b>Pinellia Rhizoma is prepared with ethanol. The orthogonal experimental design was adopted for investigating amount of ethanol, preparing time, ethanol concentration and preparing temperature. The optimal technology was determined by the comprehensive score of toxicological indicators of PGE2 content of rat celiac percolate, with the rabbit conjunctival irritation test as the intuitive validation on toxicology reduction. The pharmacodynamics validation was used to determine the reasonability of the preparation process.</p><p><b>RESULT</b>The optimal technology was that Pinellia Rhizoma was prepared by 75% ethanol at the temperature of 60 degrees C by 4 days, and then dried. The effect of relieving cough, reducing sputum and anti-inflammatory of Pinellia Rhizoma is not reduced after prepared by ethanol.</p><p><b>CONCLUSION</b>The optimal technology of Pinellia Rhizoma prepared by ethanol is simple and reasonable that it can be used as the new method to reduce toxicity and keep efficacy of Pinellia Rhizoma.</p>