Thin-layer chromatographic method for estimation of gamma-aminobutyric acid from brain.

The present method describes the estimation of gamma-aminobutyric acid (GABA) from adult rat brain by thin layer chromatography (TLC). The major advantage of this TLC method is the sensitivity and repidity of estimation. About 93 to 98 percent recovery is possible by this procedure and this shows linearity up to 9 micrograms. Replicate analysis of GABA by this method shows a co-efficient variation of 1.31%