ABSTRACT
Background: India is underperforming when it comes to compliance to hand hygiene. Early education on followed by regular hand hygiene audits can bring positive changes in infection control practices.Methods: Group I included the 3rd Semester MBBS students who had early education and training on hand hygiene and Group II included the post graduate residents who were exposed to Hand Hygiene later in their carrier were followed up for compliance of WHO Hand Hygiene moments for four months. Compliance among students and residents were observed and compared.Results: Compliance rate was more (40.4%) among medical students as compared to residents (17%). The After moments had a better compliance than Before moments among both students and residents. The difference in the compliance rate was statistically significant.Conclusions: Early clinical exposure of the students to any problem, is the key for better compliance thus explaining the better compliance rate among medical students. Better HH practice can bring down prevalence of Healthcare associated infection by multidrug resistant organism which is a major concern today.
ABSTRACT
Background: AmpC beta lactamases are cephalosporinases that confer resistance to a wide range of beta lactam drugs thereby causing serious therapeautic problem. As there are no CLSI guidelines for detection of AmpC mediated resistance in Gram negative clinical isolates and it may pose a problem due to misleading results, especially so in phenotypic tests. Although cefoxitin resistance is used as a screening test, it does not reliably indicate AmpC production. Materials and Methods: We planned a study to determine the occurrence of AmpC beta lactamase in hospital and community, clinical isolates of Escherichia coli and simultaneously evaluate different phenotypic methods for detection of AmpC beta lactamases. Results: It was observed that 82.76% isolates were ESBL positive and 59% were cefoxitin screen positive. Using phenotypic confi rmatory tests the occurrence of Amp C beta lactamases was found to be 40% and 39% by inhibitor based method using boronic acid (IBM) and modifi ed three dimensional test (M3D) respectively. Conclusion: Both the test showed concordant result. Co-production was observed in 84.62% isolates Screening of ESBL and Amp C can be done in routine clinical microbiology laboratory using aztreonam and IBM respectively as it is a simple, rapid and technically less demanding procedure which can be used in all clinical laboratories.
ABSTRACT
Small, white, thread-like, motile worms were recovered from the conjunctival sac of a 13-year-old girl and a 50-year-old woman from Dibrugarh district, Assam, India. They were identified as thelazia species. These two cases have been reported due to their rarity in India and elsewhere.