ABSTRACT
An enzyme-linked immunosorbent assay (ELISA) was evaluated for serodiagnosis of human ocular and visceral gnathostomiasis in comparison to an indirect haemagglutination (IHA) and precipitin (PPT) tests. The ELISA antibody titers were found to range from 1:400 to 1:51,200 against somatic and 1:200 to 1:25,600 against excretory-secretory (ES) antigens. When sera were tested at single dilutions, the ELISA was positive in 7 of 8 gnathostomiasis cases while only 5 and 3 were positive by IHA and PPT respectively. The overall specificity of the ELISA was 96.7% and 97.4% with somatic and ES antigens respectively. Since somatic and ES antigens produced similar ELISA results, either can be used for diagnostic purpose. It was suggested that the ELISA was a reliable serodiagnostic test for human gnathostomiasis.
Subject(s)
Animals , Antigens, Helminth/analysis , Enzyme-Linked Immunosorbent Assay , Gnathostoma/immunology , Hemagglutination Tests , Humans , Nematode Infections/diagnosis , Precipitin TestsABSTRACT
Five hundred sera from blood donors were examined for antibodies to Toxoplasma by the indirect hemagglutination antibody (IHA) and the indirect fluorescent antibody (IFA) techniques. In addition, the IHA test was used to detect Toxoplasma antibodies in 500 pregnant women's sera. It was found that 4.6% of the blood donors were positive by the IHA and only 1.2% by the IFA methods. The seroprevalence, however, was less in pregnant women as only 2.8% were positive. Interestingly, 7.5% of the blood donors and 15.2% of pregnant women showed no detectable antibodies. The frequency distribution curves of IHA titers were unimodal in both groups studied. From the basis of these findings, it was concluded that there was a low degree of Toxoplasma transmission in Chiang Mai.