ABSTRACT
Background and objective: Intrauterine insemination (IUI) after ovarian stimulation with clomiphene citrate has been a mainstay of therapy for couples suffering from non-tubal infertility because IUI is a simple, non-invasive, and cost-effective technique. Pregnancy rates after IUI were 8% to 15% on the average. Although the number of available follicles was increased by ovarian stimulation, the results are still not very promise. Because the clinical pregnancy rate of IUI in Srinagarind Hospital was 3.08%. The new insemination method for improving the clinical pregnancy rate was considered to be an alternative way technique which could be reduced cost and duration of treatment and avoid complication of the assisted reproductive technology. A preliminary RCT compared the clinical pregnancy rate of IUTPI and IUI was the main objective of the study.Methods: The study was conducted from April 1, 2007 to March 31, 2008 at Infertility clinic, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University. The volunteers were women with non-tubal infertility who met the inclusion criteria (unexplained infertility, minimal-mild endometriosis, mild oligozoospermia) and undergoing first treatment cycle were randomized to the study. Twenty eight women were randomized to the study and allocation to group A (IUTPI) or group B (IUI) on the day of insemination followed the ovarian stimulation with clomiphene citate (CC). On the day of 36-40 hours after hCG administration, IUTPI was performed by using a pediatric Foley's catheter size 8 F inseminating with 10 mL of sperm suspension and IUI was performed with 0.5 mL of sperm suspension by using the household IUI catheter.Results: A total of 28 cycles was completed treatment and followed up (16 IUTPI cycles and 12 IUI cycles).The clinical pregnancy rate per cycle was 6.3%(1/16) for IUTPI and 0%(0/12) for IUI, p=1.00. The ongoing pregnancy rate per cycle was 6.3%(1/16) for IUTPI and 0%(0/12) for IUI, p=1.00. No multiple pregnancy, ectopic pregnancy, ovarian hyperstimulation syndrome and infection were occurred.Conclusion: This preliminary result of IUTPI after ovarian stimulation with clomiphene citrate might not be advantage over the standard IUI for couples with non-tubal infertility on pregnancy rate.Keywords: Intrauterine tuboperitoneal insemination, intrauterine insemination, clinical pregnancy rate, ongoing pregnancy rate
ABSTRACT
no abstract
ABSTRACT
Zinc is an essential micronutrient for spermatogenesis and normal sperm function. However, the correlation of zinc level in seminal plasma and the results of semen analysis is controversial. This study aimed to evaluate zinc levels in seminal plasma of infertile men. One hundred thirty five left-over semen of infertile men collected from the Reproductive Biology Unit, Department of Obsterics and Gynecology, Faculty of Medicine, KKU were analyzed for semen analysis. The seminal plasma was then classified into 2 groups according to its results of semen analysis. Normal group was named normozoospermia (n = 61). The samples classified as oligospermia, asthenozoospermia, oligoasthenozoospermia, and azoospermia were included in abnormal group (n = 74). They were used for zinc determination by atomic absorption spectrophotometer. Zinc levels of normal and abnormal group were 14.21 mg% and 14.72 mg% , respectively. Mean values of zinc level in two groups were significantly different (P \< 0.001) with 95 % CI of 11.75-16.68 mg% for normal group, and of 12.64-16.81 mg% for abnormal group. The results implied that determination of zinc level in seminal plasma of infertile men might not be very useful due to a miniscule difference mean value of this ion in both groups. Further study of zinc contents should be investigated in subgroup of semen analysis, or in comparing of fertile and infertile men.
ABSTRACT
Objective : To assess in-vitro fertilization rate of human oocytes at reproductive biology unit in Srinagarind HospitalDesign : A descriptive studySetting : Reproductive biology unit, Srinagarind Hospital, Khon Kaen University, Khon Kaen, ThailandSubject : Total of 476 human oocytes undergone in-vitro fertilization at Srinagarind Hospital between May 1993 to December 2004Results : Total of 476 human oocytes were retrieved from 109 cycles of 89 patients. The average number of oocytes obtained per retrieval was 4.5 + 2.5. During the study period there were 376 mature oocytes collected (79.0 %). The fertilization rate detected in this study was 50.6 % of total oocytes or 73.3 % per treatment cycle. The clevage rate of the fertilized oocytes was 95.0 % and the embryo transfer rate was 92.2 % per cycles with successful fertilization.Conclusion : This study demonstrated that in-vitro fertilization of human oocytes performed in Srinagarind Hospital had acceptable clevage rate and embryo transfer rate but the fertilization rate was rather low.