ABSTRACT
Background: Ribosomal proteins are traditionally associated with protein biosynthesis until recent studies that implicated their extraribosomal functions in human diseases and cancers. Our previous studies using GeneFishingTM DEG method and microarray revealed underexpression of three ribosomal protein genes, RPS26, RPS27, and RPL32 in cancer of the nasopharynx. Herein, we investigated the expression pattern and nucleotide sequence integrity of these genes in nasopharyngeal carcinoma to further delineate their involvement in tumourigenesis. The relationship of expression level with clinicopathologic factors was also statistically studied. Methods: Quantitative Polymerase Chain Reaction was performed on nasopharyngeal carcinoma and their paired normal tissues. Expression and sequence of these three genes were analysed. Results: All three ribosomal protein genes showed no significant difference in transcript expressions and no association could be established with clinicopathologic factors studied. No nucleotide aberrancy was detected in the coding regions of these genes. Conclusion: There is no early evidence to substantiate possible involvement of RPS26, RPS27, and RPL32 genes in NPC tumourigenesis.
ABSTRACT
Using a questionnaire, a snoring study was done in 400 medical and 78 nursing students of University Malaysia Sarawak in 2004 and 2005. The response rates were 72.5% for medical students and 83.3% for nursing students. The habitual snoring percentages for all years, first year and final year medical students were 7.2, 9.9 and 3.6 respectively; and for all years, first year and third year nursing students were 12.3, 13.3 and 8.3. These figures were lower than those found in Hong Kong and Germany, despite similar body mass index (BMI). In the combined group of student, positive correlation with snoring was found in male gender (p=0.011), BMI (p=0.002) and sleep apnoea (p=0.05). For medical students, positive correlation was found in male gender (p<0.0005), weight (p <0.001), height (p <0.001) and sleep apnoea (p<0.001), and for nursing students in BMI (p=0.008) and weight (p=0.033). No significant correlation was found in snoring with Epworth Sleepiness Scale and academic performance.