ABSTRACT
Aim To establish a method for determining the antithrombotic biological activity of Honghua injection, which can be used to evaluate and control its quality. Methods Collagen-adrenalin was used to induce mouse acute cerebral thrombosis model and hemiplegic protection rate of Honghua injection in mice as an index to investigate the antithrombotic activity of Honghua injection. The experimental conditions of the administration dosage, inducer dosage, and different strains of mice were investigated, and the experimental conditions were optimized by orthogonal design. Results Honghua injection which was made by 5. 0 g crude drug · kg-1and continuously ip for 3 d, had obvious protective effect on collagen-adrenalin-induced acute cerebral thrombosis in mice. The established biological activity limit method showed a good repeatability, intermediate precision, and reproducibility. Each sample showed different degrees of differences in biological activity. Conclusions The thrombus test in mice can be used as a method for measuring the biological activity of Honghua injection. It is recommended to use 5. 0 g crude drug · kg-1dose as the limit for bioactive process optimization and product quality controlment of Honghua injection.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of xue-bao capsules on injury of radio-or chemo-therapy in mice, in order to provide rationale behind clinical trials.</p><p><b>METHOD</b>xue-xu (deficiency of blood) model in mice was induced by radiation or cyclophosphamide. Leucocyte (WBC), erythrocyte (RBC), hemoglobin (Hb) and platelet (Pt) in peripheral blood as well as CFU-E and CFU-Gm in bone marrow were counted.</p><p><b>RESULT</b>CFU-E and CFU-Gm in normal mice were promoted by this drug. The reduction of WBC, RBC and Hb in peripheral blood as well as CFU-E and CFU-Gm in bone marrow owing to the 3.5 Gy of 60Co radiation were antagonized by the drug. It had also antagonized cyclophosphamide induced the reduction of WBC, RBC and Pt in peripheral blood.</p><p><b>CONCLUSION</b>xue-bao capsules has the effects against the adverse reactions of radio-or-chemo-therapy.</p>
Subject(s)
Animals , Female , Male , Mice , Bone Marrow Cells , Cell Biology , Radiation Effects , Capsules , Cell Count , Cells, Cultured , Cyclophosphamide , Toxicity , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Erythrocyte Count , Erythroid Precursor Cells , Radiation Effects , Granulocyte Precursor Cells , Radiation Effects , Leukocyte Count , Plants, Medicinal , Chemistry , Platelet Count , Radiation Injuries, Experimental , Blood , Pathology , Random Allocation , Whole-Body IrradiationABSTRACT
<p><b>OBJECTIVE</b>Using animal anemia models to observe the antianemia effect of Shengxuesu, and to afford an experimental basis for preventing and treating anemia.</p><p><b>METHOD</b>Rat model of iron deficiency induced by denutrition and mouse model of nemorrhagic anemia by blood lefting were established. Indices of hemoglobin(HB), red blood cell count(RBC), hematocrit(HCT), mean corpuscular hemoglobin count(MCHC), serum iron(SI), serum ferritin (SF) and total iron-binding capacity(TIBC) were monitored.</p><p><b>RESULT</b>A dosage of Shengxuesu 0.5-2 g.kg-1 was given to the rat model of hypoferric anemia by gavage for 15 days, and to the mouse model of hemorrhagic anemia by gavage for 7 days. The result shows that HB, RBC, HCT, MCHC in blood and iron, ferroprotein in serum were elevated significantly; but total bounding iron in serum was decreased. Meanwhile, diet amount, diet consumption and general activity of the model rats were increased.</p>
Subject(s)
Animals , Female , Male , Mice , Rats , Anemia, Hemolytic , Blood , Anemia, Iron-Deficiency , Blood , Atractylodes , Chemistry , Capsules , Deer , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Erythrocyte Count , Hematocrit , Hemoglobins , Metabolism , Iron , Blood , Materia Medica , Pharmacology , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>Various models of experimental myocardial ischemia were set up to ovbserve the Yixinton's protection against ischemia.</p><p><b>METHOD</b>Models of experimentally acute myocardial ischemia were made by ligature or medication (pituitrin or isoprenaline) to check the indices of ECG, hemodynamics, and morphology.</p><p><b>RESULT</b>A dosage of Yixintong tablets 100 mg/kg, 200 mg/kg was given to rats by gavage; the rats had undergone a thirty-minute ligatute of coronary lef anterior descending branch, and used as a model of ischemic reperfusion. The observations showed that Yixintong tablets exerted a recovery effect on heart rate, blood pressure, internal pressure of left ventricule and its peak/trough rate (+/- dp/dtmax), and ST segment (electrocardiogram). The tablet markedly reduced the myocardial infarct size of the coronary-ligatured rats. The tablet benefited the rats with pituitrin(iv)- or isoprenaline(ip)-induced acute myocardial ischemia to reverse any ST deviation and T-wave fall.</p><p><b>CONCLUSION</b>Yixintong has a protective and therapeutic action to the experimental myocardial ischemia.</p>