ABSTRACT
【Objective】 To explore the clinical applications of low ionic strength salt solution polyethylene glycol (LISS-PEG) and low ionic strength salt solution bovine serum albumin (LISS-BSA) in the indirect antiglobulin test (IAT). 【Methods】 The common standard red blood cell(RBC) IgG irregular antibodies (anti-D, anti-M, anti-N, anti-S, anti-s, anti-Jka, anti-Jkb, anti-Fya, anti-Fyb, anti-Dia, anti-K) were reacted with RBCs with corresponding antigens in IAT with LISS-PEG and LISS-BSA modification for 5-minute and 10-minute incubations, and then compared the results with conventional IAT. One hundred of blood samples from patients presenting irregular antibodies of erythrocyte IgG were selected to observe the effect of these two self-made modification. The agglutination intensity was recorded by AABB scoring method. 【Results】 No difference was noticed in IAT intensity reaction between LISS-PEG 5-minute and 15-minute incubation (P>0.05), nor between 5-minute/15-minute LISS-BSA incubation and conventional IAT (P>0.05). However, LISS-PEG modification demonstrated a significant superiority over the conventional technique just after incubation with 5 minutes(P<0.05). 【Conclusion】 Using the self-made LISS-PEG as the enhancement medium allows not only reduced incubation time (5 minutes) but also increased intensity of the reaction, which shortens the cross-matching time for emergency blood transfusion and is worthy of popularization.
ABSTRACT
Objective To investigate the effect of microcolumn gel reagent card,micro-column glass bead reagent card and classical test tube on the antibody titer of O-type pregnant women with ABO blood group.Determination of antibody potency of pregnant women with rabbit by reagent card.Methods Using microcolumn gel reagent card,micro-column glass beads reagent card,and classical test tube parallel detection to detect the IgG blood group antibody titer of O blood type pregnant women.Results There was no significant difference in the positive detection rate between the microcolumn gel reagent card and the microcolumn glass beads reagent card at 1:128 and the test tube method at 1:64 (P＞0.05).Conclusion The clinical reference value of microcolumn gel reagent card and microcolumn glass beads reagent kit for detecting antibody titer of IgG blood group in O-type pregnant women should be set to ≥ 1:128.