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Objective To investigate the pressing time and puncture site bleeding after extubation of superficial venous catheter in elderly patients.Methods Four hundred elderly inpatients were selected from respiratory department during June 2015 and December 2015 and were divided into two groups:200 patients as experimental group with pressing time of 5-6 minutes and 200 patients as control group with pressing time of 3-4 minutes.The same group of nurses pressed the puncture site after extubation and changes on skin and vessels were observed,which was to find out the optimal pressing time after extubation of superficial venous catheter in elderly patients.Results The presence of bleeding,bruising,success rate of the second puncture,swelling,pain and degree of comfort were compared between two groups and significant differences were found (P＜0.05).Conclusion Compared with those in control group,less bleeding,bruising,swelling,and pain,higher success rate of the second puncture and lower degree of comfort were found in experimental group.
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OBJECTIVE: To establish the quality standard of Tongtong granule. METHODS: Panax notoginseng,Ginkgo biloba and Pueraria lobata were identified qualitatively by TLC and the content of total flavonol glyconsides in G. biloba was determined by HPLC. RESULTS: The TLC spots were distinctive and specific. The linear range of quercetin, kaempferide, isorhamnetin were 5.24～83.84 ?g?mL-1(r=0.999 2), 4.56～72.96 ?g?mL-1(r=0.999 1) and 2.48～39.68 ?g?mL-1(r=0.999 4), respectively. The average recoveries were 96.21%(RSD=2.06%)、99.14%(RSD=1.19%)、95.00%(RSD=1.31%),respectively. CONCLUSION:The method is reliable, accurate, and reproducible for the quality control of Tongtong granule.
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AIM:To establish the method of determining sodium new houttuyfonate in plasma and study its pharmacokinetics in beagle dogs.METHODS:The HPLC was employed with chromatographic conditions,such as octa-silicane bond silica gel column(250 mm?4.6 mm,5 ?m)as analytical column,methanol-water-ammonium hydroxide with 4 butyls(70 ∶30 ∶0.3)as mobile phase and at the flow-rate of 1.0 mL/min.The UV detection was set at 286 nm.The pharmacokinetic parameters were calculated by DAS(ver 1.0)program and compared by statistic analysis.RESULTS:The calibration curve was linear in blood plasma over the concentration range of 0.128～25.575 mg/L(r= 0.999 2),good precision and high recoveries.The parameters were T_ max= 5.0 min,C_ max= 4.982 mg/L,T_ 1/2=8.67 min.CONCLUSION:The analytical method established in this study is sensitive and exact,and can be used to measure the pharmacokinetics of sodium new houttuyfonate in beagle dog.The sodium new houttuyfonate can metabolize quickly following intravenous injection.