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Objective To study the expression of nicotinamide phosphoribosyl transferase (Nampt) in breast cancer and to investigate the effects of Nampt inhibitor on the growth and chemotherapy sensitivity of breast cancer cells.Methods Nampt mRNA expression in breast cancer tissues and adjacent normal tissues was detected by qRT-PCR method.The effect of Nampt inhibitor on the growth of breast cancer cells and chemotherapy sensitization was detected by MTT and soft agar clonogenic assays.Results Nampt in breast cancer was significantly higher than that in normal breast tissue (P=0.000).Nampt inhibitor FK866 repressed breast cancer cell proliferation (24 h,P=0.003;48 h,P=0.001) and suppressed cell anchorage-independent growth (0.3 nM vs 0 nM,P=0.02;3 nM vs 0.3 nM,P=0.0143;0 nM vs 3 nM,P=0.02).FK866 also increased the chemo-sensitivity of breast cancer cells to fluorouracil by greater inhibition of cell proliferation.Conclusion Our findings indicate that Nampt may be a new therapeutic target for breast cancer.
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Objective To conduct a meta-analysis to explore the merits and disadvantage of total endoscopic thyroidectomy and conventional open thyriodectomy.Methods A database searching was performed on the Chinese BioMedical Literature on disc(CBM),China National Knowledge Infrastructure(CNKI),wanfang database,VIP database and PubMed database.Prospective randomized controlled studies about total endoscopic thyroidectomy and conventional open thyroidectomy were selected and analyzed.RevMan 5.2 was used for statistical analysis.Results Eleven studies matched the selection criteria,which involved 1082 patients,among whom 505 patients underwent total endoscopic thyrodiectomy (endoscopy group) and 577 patients underwent conventional open thyroidectomy (open group).Outcomes of total endoscopy group and open group in satisfaction,operation time,drainage fluid,complications and post-operative hospital stay etc were compared.Meta analysis showed endoscopy group were supeior to open group in terms of intraoperative blood loss (WMD＝-17.86,95％ CI:[-26.18,-9.54]),postoperative hospital stay (WMD＝-0.95,95％ CI:[-1.40,-0.50])and patients' satisfaction (WMD＝4.03,95％ CI:[1.20,13.58])while the operation time was longer in endoscopy group than in open group (WMD＝12.61,95％ CI:[2.85,22.38]).There was no statistical significance on postoperative drainage(WMD＝ -0.70,95％ CI:[-32.79,31.39]) or complications (RR＝0.84,95％ CI:[0.54,1.29])for the two groups.Conclusions Total endoscopic thyriodectomy has advantages in terms of satisfaction,post-operative hospital stay,and intraopetative blood loss while it takes more operation time.There is no difference among drainage fluid or complication.
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Objective To compare and evaluate the efficacy of diagnosis and excision for appropriately selected breast multi-focal lesions and solitary lesion by ultrasound-guided vacuum-assisted biopsy (UGVAB). Methods Among 392 appropriately selected patients, 187 patients with multi-focal lesions and 205 patients with solitary lesion were treated by the 8-gauge UGVAB from May 2007 to June 2009. All lesions were removed as completely as possible. The patients with benign pathology underwent physical and ultrasound examinations at one week and 6 months after procedure. Results During the procedure, only three patients had vasovagal syncope and twenty others complained of other intraoperative discomfort. An accurate pathological diagnosis was obtained in all lesions. There was no apparent false-negative result among the 696 lesions with benign pathology at a follow-up of 6 months after procedure. The rates of malignant or premalignant pathology, postoperative complications and residual lesions in patients with multi-focal lesions were higher than those in patients with solitary lesion. If each lesion was considered as a subject of study, there was no significant difference between the two groups. Conclusion UGVAB is an effective method for diagnosis and excision of appropriately selected breast multi-focal lesions and can be used routinely.