ABSTRACT
<p><b>OBJECTIVE</b>The synergic and decreasing toxic effects of mineral water and Chinese herbal compound preparation (MWCHCP) on cisplatin were investigated in sarcoma 180 (S180) mice.</p><p><b>METHOD</b>The S180 mice were treated for 5 days with intraperitoneal injection of cisplatin(7.33 mg x kg(-1)) and oral administration of MWCHCP(1 925, 3 850, 7 700 mg x kg(-1)). Then the mice were killed and the tumor growth inhibition rate, organ index, diarrhea index were determined. Observe pathological sections of stomach to study the protective effect of MWCHCP. Reverse transcription-polymerase chain reaction (RT-PCR) was applied to investigate the tumour necrosis factor-alpha (TNF-alpha) expression level of the intestine.</p><p><b>RESULT</b>Combining with cisplatin and MWCHCP caused a tendency of increasing the tumor growth inhibition rate and significant attenution of cisplatin-induced diarrhea, visceral organ injury, gastric mucosal injury and decreased TNF-alpha mRNA level of intestine.</p><p><b>CONCLUSION</b>The present findings suggest that MWCHCP increases the inhibition rate of tumor growth of cisplatin and has a beneficial influence on gastrointestinal lesion induced by cisplatin.</p>
Subject(s)
Animals , Humans , Male , Mice , Antineoplastic Agents , Cisplatin , Disease Models, Animal , Drug Synergism , Drug Therapy, Combination , Drug-Related Side Effects and Adverse Reactions , Drugs, Chinese Herbal , Mineral Waters , Sarcoma 180 , Drug Therapy , PathologyABSTRACT
Three hundred and one piglets of Suzh ong line 1 and 2 were chosen for halothane test(HT), with the halothane positive rate of 1.33%. 34 halothane negative(HN) piglets were randomly selected for gen otype testing and 6 of them were found to be heterozygous. The results showed th at genotype frequencies of HalNN, HalNnand Halnn w ere 81.26 %, 17.4% and 1.33%, respectively, while the frequencies of N gene and n gene wer e 89.97% and 10.03%, separately. 4 pigs with genotype Halnn gave worst meat quality appearing 3 typical and 1 slight PSE meat. Pigs with genotype Hal N Nor HalNndid not show PSE meat. 208 offspring from 20 HN sows mat ed wit h halothane positive(HP) boars, 21 piglets from 4 litters were HP, with low dail y gain, low feed efficiency and bad meat quality but high dressing percentage, l arge loin-eye area and high lean meat percentage.