ABSTRACT
This study aimed to provide a scientific basis for the application of the mycorrhizal planting technology of Dendrobium officinale by investigating the effects of mycorrhizal planting on the fingerprints of D. officinale and the content of six chemical components. Seventeen samples of D. officinale under mycorrhizal and conventional planting were collected from four regions, such as Jinhua of Zhejiang. The HPLC fingerprints were established to evaluate the similarity of the samples. The content of six chemical components of the samples was determined by HPLC. There were 15 common peaks in the fingerprints, and five of them were identified by marker compounds, which were naringenin, 4,4'-dihydroxy-3,5-dimethoxybibenzyl, 3,4'-dihydroxy-5-methoxybibenzyl, 3',4-dihydroxy-3,5'-dimethoxybibenzyl(gigantol), and 3,4-dihydroxy-4',5-dimethoxybibenzyl(DDB-2). The similarities of the fingerprints of mycorrhizal and conventional planting samples and the control fingerprint were in the ranges of 0.733-0.936 and 0.834-0.942, respectively. The influences of mycorrhizal planting on fingerprints were related to planting regions, the germplasm of D. officianle, and the amount of fungal agent. The content of six chemical components in the samples varied greatly, and the content of DDB-2 was the highest, ranging from 69.83 to 488.47 μg·g~(-1). The mycorrhizal planting samples from Chongming of Shanghai and Taizhou of Jiangsu showed an increase in the content of 5-6 components, while samples from Zhangzhou of Fujian and Jinhua of Zhejiang showed an increase in the content of 1-2 components. The results showed that mycorrhizal planting technology did not change the chemical profile of small molecular chemical components of D. officinale, but affected the content of chemical components such as bibenzyls, which has a good application prospect.
Subject(s)
Dendrobium/chemistry , Mycorrhizae , China , Chromatography, High Pressure LiquidABSTRACT
Dendrobium officinale is a member of the family Orchidaceae. The dried stem of D. officinale is used as a valuable traditional Chinese medicine, known as Dendrobii Officamlis Caulis (called TiepiShihu in Chinese). According to Chinese Pharmacopoeia, Dendrobii Officamlis Caulis has effects of tonifying stomach, promoting fluid, nourishing Yin and clearing heat. At present, the planting area of D. officinale is over 100 000 Mu (over 6 670 hm2) and the annual output of its fresh stem is in excess of 10 000 tons. Good variety is the guarantee of herbal medicine's quality, while germplasm resource is the base for breeding excellent variety. In this paper, we summed the characteristics of present main varieties of D. officinale and reviewed the progress on germplasm resources and genetics and breeding of the plant, in order to provide a scientific basis for the further research.