ABSTRACT
Objective: To investigate the changes of energy metabolism before and after ginger-processing of Coptidis Rhizoma, and elucidate the relationship between the changes of medicinal properties and the energy metabolism. Methods: Crude Coptidis Rhizoma and ginger-processed Coptidis Rhizoma decoctions were prepared and administered to rats once a day for 7 d by gavage. Blood and liver were used to detect the content and activity of key enzymes in three pathways of energy metabolism. The composition analysis analyzed the detection results and screened out the key enzymes for the main changes in energy metabolism before and after processing. Results: Compared with the control group, the activities of HK, PFK, PK and mitochondrial respiratory chain complex I, II, III and IV in crude Coptidis Rhizoma group were decreased significantly, and the content of CS, ICD and α-KGDH was decreased significantly.The activities of mitochondrial respiratory chain complexes II, III and IV and the activities of PFK and PK in the ginger-processed Coptidis Rhizoma group were significantly decreased. HK and mitochondrial respiratory chain complex I was decreased to some extent, but there was no significant difference. The content of α-KGDH was decreased significantly, and the content of CS and ICD was decreased in a certain degree. Compared with the crude Coptidis Rhizoma group, the activities of HK, PFK, PK and mitochondrial respiratory chain complex I, II, and III in the ginger-processed Coptidis Rhizoma group were increased significantly, and the content of CS, ICD and α-KGDH was increased significantly. There was no significant change in the mitochondrial respiratory chain complex IV. Two principal components were extracted by principal component analysis. Among them, complex II and complex III had the maximum contribution rate of principal component 2 and principal component 1 of 0.916 and 0.873, respectively. Conclusion: The weakening of cold in nature of Coptis chinensis after ginger-processed treatment may be due to the inhibition of the inhibitory effect of C. chinensis on mitochondria respiratory chain complex II and complex III in the oxidative phosphorylation pathway.
ABSTRACT
Objective: To investigate the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats and explore its therapeutic mechanism,and compare the difference of this herb before and after simmering. Method: Healthy SD rats were randomly divided into 7 groups,including the blank group,the high,medium and low dose groups of raw Rhei Radix et Rhizoma(1.5,3.0,6.0 g·kg-1·d-1) and the high,medium and low dose groups of simmered Rhei Radix et Rhizoma(1.5,3.0,6.0 g·kg-1·d-1),eight rats in each group were continuous intragastric administration for 10 days,the effect of Rhei Radix et Rhizoma on gastrointestinal function of rats were evaluated by body weight,food intake,gastric remnant rate,small intestine propelling rate,stomach weight coefficient and gastrointestinal tissue morphology;enzyme-linked immunosorbent assay(ELISA) was used to detect contents of motilin(MTL) in rat plasma and gastrin(GAS),tumor necrosis factor-α(TNF-α),interleukin-10(IL-10) in rat serum,the mechanism of effect of Rhei Radix et Rhizoma on gastrointestinal function was explored and the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats before and after simmering was compared. Result: In each administration group,weight growth of rats was slowed down,gastric emptying and intestinal propulsion were inhibited,inflammatory reaction was triggered,and gastric mucosal injury was increased.Compared with the blank group, except for the low-dose and medium-dose groups of simmered Rhei Radix et Rhizoma,stomach weight coefficient,gastric remnant rate and content of TNF-α of rats were significantly increased(PPPPα of rats were all decreased to different degrees. Conclusion: Gastrointestinal dysfunction caused by Rhei Radix et Rhizoma is related to inhibiting the expression of gastrointestinal hormones and promoting the expression of inflammatory cytokines, in addition,the effect of "stomach injured by bitterness and cold property" of Rhei Radix et Rhizoma is weakened after simmering,which provides scientific basis for clinical rational use of Rhei Radix et Rhizoma.