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To summarize the experience in treating reverse psoriasis based on location-based syndrome differentiation. It is believed that the main pathological factors in the onset of reverse psoriasis are dampness， heat， stasis， and toxins. In clinical practice， treatment is tailored based on the location-based syndrome differentiation and treatment according to the presence of dampness， heat， stasis， and toxins. For cases that manifest predominantly in the upper body with wind-heat attacking the surface， the treatment focuses on clearing heat， dispersing wind， and relieving itching， and a self-designed Sanhua Decoction （三花汤） is used. Alternatively， for cases with blood heat accumulating and stagnation， the treatment emphasizes on clearing heat and toxins， and cooling blood to eliminate skin lesions， and self-designed Sancao Decoction （三草汤） is employed. For cases that mainly affect the middle part of the body with damp-heat stagnating in the spleen， the treatment focuses on clearing heat， resolving toxins， and drying dampness while invigorating the spleen， and a self-designed Sanhuang Decoction （三黄汤） is applied. For cases with stasis and heat intertwining， the treatment aims to resolve stasis， clear heat， and activate collaterals while detoxifying， and a self-designed Santeng Decoction （三藤汤） is used. For cases that predominantly affect the lower part of the body with damp-heat descending， the treatment focuses on detoxification， eliminating dampness， and clearing and promoting the lower jiao， and a self-made Sanling Decoction （三苓汤） is used. For cases with cold and dampness accumulating and toxins， the treatment emphasizes on warming yang， supplementing qi， and detoxification while eliminating dampness， and a self-made Sanshen Decoction （三参汤） is prescribed.
ABSTRACT
Objective To provide references for the clinical application of Dunhuang prescriptions for treating internal medicine diseases by analyzing medication rules of Dunhuang manuscriptsFu Xing Jue based on data mining method.Methods TCM prescriptions for internal medicine diseases in the Dunhuang manuscriptsFu Xing Jue were input computer. Excel 2003 software was used to establish relevant database. Data mining method was used to analyze the medication rules.Results There were 61 TCM prescriptions in the Dunhuang manuscriptsFu Xing Jue, including66 kinds of Chinese herbal medicine and 336 times of total frequency of usage. The used core single herbs were as follows: Glycyrrhizae Radix et Rhizoma, Paeoniae Radix Alba, Zingiberis Rhizoma,Zingiberis Rhizoma Recens, Scuteliariae Radix, Inulae Flos, Ginseng Radix et Rhizoma, Jujubae Fructus, Lophatheri Herba, Schisandrae Chinensis Fructus, and Cinnamomi Ramulus; medicine types were tonifying-deficiency medicine, heat-clearing medicine, relieving exterior syndrome medicine, interior-warming medicine and antitussive and antiasthmatic medicine, with the cumulative frequency of 80.66%; medicine flavors were bitterness, sweetness and pungentness, with the cumulative frequency of 83.91%; medicine properties were coldness, warmness and peace, with the cumulative frequency of 87.95%; channel tropisms were stomach, lung, spleen, heart, kidney and liver, with the cumulative frequency of 86.15%. Clinical compatibility of medicines for treating internal medicine diseases in Dunhuang medical prescriptions were mainly qi-tonifying medicines (Glycyrrhizae Radix et Rhizoma, Ginseng Radix et Rhizoma and Jujubae Fructus), blood-replenishing medicine (Paeoniae Radix Alba), and nourishing medicines (Ophiopogonis Radix), supplemented by heat-clearing medicine (Lophatheri Herba andScuteliariae Radix),with relieving exterior syndrome medicines (Zingiberis Rhizoma Recens andCinnamomi Ramulus),interior-warming medicine (Zingiberis Rhizoma), antitussive and antiasthmatic medicine (Inulae Flos), inducing astringency medicine (Schisandrae Chinensis Fructus) as characteristic medication. High-frequency medicines constitute Dunhuang medical prescription of Xiaoyindan Decoction, which can be used to treat epidemic. Conclusion This article concluded the compatibility rules of Dunhuang manuscripts Fu Xing Jue, provide references for clinical medication and research and development of new medicine.
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Objective To study the medication rule of traditional Chinese medicine treatment of urinary stone disease data mining. Methods Clinical study literature in China Journal Full-text Database 1985-2012 on Chinese medicine prescription of urinary stone disease were retrieved, database was established and analyzed by SPSS12.0 statistical software. Results There were 209 literatures meeting the inclusion criteria of Chinese medicine for urinary lithiasis, including 209 oral administration compound, 234 kinds of traditional Chinese medicine, 1843 times of frequency. The traditional Chinese medicine mainly for diuresis and diffusing dampness, restoring vital energy, blood-activating and removing stasis, regulating qi, clearing heat, promoting digestion and purgation, counted for 86.43%;with flavour of sweet, bitter, spicy, fresh, counted for 86.67%;nature of cold, plain, warm, counted for 93.08%;meridian tropism of liver, bladder, spleen, stomach, kidney, lung, heart, counted for 83.67%. Conclusion Trditional Chinese medicine of diuresis and diffusing dampness, restoring vital energy, blood-activating and removing stasis, regulating qi, clearing heat were commonly used. The core single medicine are Lysimachiae Herba, Glycyrrhizae Radix, Talcum, Lygodii Spora, Plantaginis Semen, Pyrrosiae Folium, Cyathulae Radix, Malvae Fructus, Galli Gigerii Endothelium Corneum, Akebiae caulis, etc.
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The cDNA encoding the rabbit metallothionein-I was amplified by RT-PCR from the rabbit liver induced by cadmium and cloned into prokaryotic fusion expression vector pQE40. Then it was transformed into Escherichia coli M15. Positive expression clones were detected by colony blotting. Target protein solubility was determined by Western blotting analysis. The optimal induction condition of the level of protein expression with IPTG induction was established by SDS-PAGE electrophoresis and ImageMaster VDS software analysis. The fusion protein can be purified from lysates with Ni-NTA agarose. We found that the fusion protein with apparent molecular weight 32 KD existed in two ways: soluble and insoluble in Escherichia coli. After 1 mM IPTG induction, the level of expression of the fusion protein increased with the prolongation of induction time and reached a peak in 9 h by ImageMaster VDS software analysis, accounting for 57.4% of all the insoluble protein. The purified fusion protein was obtained by Ni-NTA affinity chromatography. This fusion protein can be used in further studies on the preparation of MT-I protein and development of protein product.