ABSTRACT
Objective:To explore the correlation between urinary phthalate concentration and semen quality parameters.Methods:A total of 112 males with infertility in our hospital were set as infertility group. Another 65 healthy males who had given birth during the same period in our hospital were selected as control group. The semen samples obtained by urine and masturbation were collected in all the subjects. The semen quality parameters were detected by automatic color sperm quality detection system, and the concentrations of dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP) and Di 2-Ethyl Hexyl Phthalate (DEHP) in urine were measured by high performance liquid chromatography, and the correlation between concentrations of four phthalates and semen quality parameters was analyzed by spearman correlation coefficient.Results:The concentrations of DMP, DEP, DBP, DEHP and total phthalate in infertility group were significantly higher than those in control group ( P<0.05). The sperm density, sperm survival rate and ratios of a+ b level sperm and forward movement in infertility group were significantly lower than those in control group ( P<0.05), and the ratio of abnormal sperm was significantly higher than that in control group ( P<0.05). The concentrations of DMP, DEP, DBP and DEHP in urine were negatively correlated with sperm density, sperm survival rate and ratios of a+ b level sperm and forward movement ( P<0.05), and were positively correlated with abnormal sperm ratio ( P<0.05). Conclusions:The concentration of phthalate is high in urine of infertile males, and its concentration is negatively correlated with semen quality. Phthalate accumulation may be one of the important reasons for male infertility.
ABSTRACT
Objective To establ ish the chromatogrphic fingerprint of Jisheng Shenqi Pills by HPLC method for evaluating the quality of the preparation. Methods The mobile phase was acetonitrile-0.3%acetic acid solution in a gradient elution mode.The flow rate was 1.0 ml/min and the UV detection wavelength was 240 nm. The similarity of 7 batches of samples was evaluated by using Similarity Calculation Software of TCM Chromatogrphic Fingerprint (2004A edition). Results Nine common peaks were identified in 7 batches of samples and found belonging to their single medicinal material. The similarity of fingerprint was all higher than 0.797. Conclusion The method is simple and accurate,reproducible for quality control of the preparation.
ABSTRACT
Objective To establish an HPLC method for determination of low-molecular-weight carbonyl compounds in the oil-containing herbs. Methods After carbonyl compounds in the samples were extracted with water, the solution reacted with 2, 4-dinitrophenylhydrazine (DNPH) in an acidic medium to form 2, 4-dinitrophenylhydrazone derivatives, which were separated on Kromasil KR100-5 C18 column (250 mm× 4.6 mm, 5 μm). The mobile phase A was water-acetonitrile-tetrahydrofuran-isopropanol (59∶30∶10∶1), mobile phase B was water-acetonitrile (35∶65), gradient elution. The flow rate was 0.8 mL/min, detection wavelength was 365 nm, and column temperature was 30 ℃. Results Good linearities were obtained in corresponding concentration ranges, with correlation coefficient over 0.999. The limits of detection of the eight DNPH derivatives were 0.002-0.008 μg/mL, and the average recoveries were 88.49%-93.65%. Conclusion The method is simple, accurate and reliable, with good reproducibility.