ABSTRACT
Objective:To study of change of Th17 cell and IL-17 caused by bacteria biofilm after infection.Methods: To detect biofilm formation ability of bacteria isolated from bronchus alveolus washing-water of patients;to detect expressing ratios of Th17 cells and IL-17 level in peripheral blood of patients and healthy person;to detect expressing ratios of Th17 cells and IL-17 level in bronchus alveolus washing-water of patients;to analyze the above data using SPSS17.0 software.Results: Expressing ratios of Th17 cells and IL-17 level in peripheral blood of patients infected by producing biofilm and non-producing biofilm bacteria and healthy person are respectively(0.59±0.18)%and(108.8±20.5)pg/ml,(0.58±0.18)%and(100.1±20.7)pg/ml,(0.55±0.17)% and(100.0± 21.4)pg/ml,they were not different among data from patients and healthy person,P>0.05,expressing ratios of Th17 cells and IL-17 level in bronchus alveolus washing-water of patients infected by producing biofilm and non-producing biofilm bacteria were respective (1.37±0.34)%,(157.4±30.8)pg/ml and(1.11±0.21)%,(136.2±24.3)mg/ml,the data between patients infected by producing biofilm and non-producing biofilm bacteria were distinctly different, P<0.05.Conclusion: Bacteria biofilm can cause Th17 cell expressing ratio and IL-17 level to become high in infected part,this may be mechanism that infection become chronic.
ABSTRACT
Objective To investigate the relationship between tumor necrosis factor-alpha(TNF-?), endothelin(ET) and Interleukin-6(IL-6) plasma level of coronary heart disease(CHD) patients and the type of the coronary plaque morphology.Methods Peripheral blood samples were taken from 230 CAG confirmed CHD patients and the same number of CAG normal patients.Serum TNF-?,ET and IL-6 values were measured through enzyme-labeled immunosorbent assay(ELISA) technique in the 2 groups and their relationship between coronary plaque morphology were assesed.Results The mean levels of serum TNF-?,ET and IL-6 in the CHD group were higher than those of the normal group(P