ABSTRACT
Background: WHO recommends that malaria drug should be used with essential elements which are derivatives of artemisinin (ART) for treatment phase and limit the development of parasite (MIC). Objective: To assess in vitro effect of artemisinin powder and 10 alpha- trifluoro methyl hydroartemisinin (TEMHA) in powder and tablet form to P.falciparum. Subject and Method: 48h in vitro test of Phuc Nguyen Dinh was applied to this study. Results and Conclusions: The results showed that: for T996, IC50 values of ART, 10 alpha- TEMHA powder and 10 alpha- TEMHA pill were as follows: 37.8; 16.4 and 17.6 nM/L, respectively. For K1, IC50 values of ART, 10 alpha- TEMHA powder and 10 alpha- TEMHA pill were: 22.8; 11.4 and 12.2 nM/L, respectively. MIC values of artemisinin powder, 10 alpha- TEMHA powder and pill for T996 were as follows: 100; 40 and 40nM/L, respectively. For K1, MIC values of ART, 10 alpha- TEMHA powder and pill are: 76; 24; 32 nM/L, respectively.
ABSTRACT
Background: In 2006, the project of global fund for malaria prevention in Vietnam provided a large number of rapid diagnostic test Paracheck F for Vietnam for the purpose of rapid malaria diagnose. However, there is no study on evaluation the effect of rapid diagnostic test compared with microscopy method. Objective: To evaluate the diagnostic yield and cost of paracheck F test and microscopy in malaria diagnosis and treatment. Subject and Method: The study was carried out in 6 communes belongs to Quang Tri and Quang Binh provinces from September to November - 2006. The study was divided into 3 phases. Phase 1: diagnoses and treatments are based on clinical symptoms, phase 2: diagnoses and treatments are based on the results of paracheck and phase 3: diagnoses and treatments are based on the results of microscopy. All phases, both the common patients and malarial patients and the amount of anti-malarial drugs were treated, the amount of money was spent on transport and days work off of malarial patients and their relatives were calculated. Result: The investigation data on expenditure of malaria patients showed that: the average direct cost of malaria patient in phase 1 is VND 116.100; phase 2: VND 119.400 and phase 3: VND 120.800 per 1 treatment course. There is no significant difference between direct costs in three phases (p > 0.05). Conclusion: The expense efficiency for finding out a case of malaria by paracheck and microscopy is equivalent and lower than the expense of diagnosis based on clinical symptoms.
Subject(s)
MicroscopyABSTRACT
Background: Paracheck F.test is rapid diagnostic test that use to determine presence of histidine rich protein 2-HPR-2, a component of Plasmodium falciparum in total blood. Paracheck F.test producted by India\r\n', u'Objective: To study the sensitivity, specificity and diagnosed conformity of Rapid Diagnosis Test (Paracheck F test) compared with microscopic method for detecting P.falciparum \r\n', u'Subject and method: The study was conducted in 5 communes of Quang Tri province and 1 commune of Quang Binh province from September to November in 2006. A total of 470 blood samples were collected and examined with 2 methods for detecting P falciparum (Microscopy and Rapid Diagnosis Test- Paracheck). \r\n', u'Results:Among these blood samples, there were 438 samples having complete conformity in both two methods. 29 samples were negative with microscopy but positive with Paracheck. 3 samples were positive with Paracheck but negative with microscopy.\r\n', u'Conclusion: the sensitivity and specificity of Paracheck is 99% and 82%, respectively. Both microscopic and Paracheck methods have a high conformity in detecting P falciparum with the Kappa index of 0.84. \r\n', u'