Antimicrobial, antioxidant activity and phytochemical prospection of Eugenia involucrata DC leaf extracts / Atividade antimicrobiana, antioxidante e prospecção fitoquímica dos extratos das folhas de Eugenia involucrata DC

The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p <0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.

A espécie Eugenia involucrata DC. é uma planta nativa do Brasil e tradicionalmente utilizada para problemas intestinais, porém, poucas pesquisas documentam sobre seu potencial biológico e perfil fitoquímico. Assim, o objetivo deste trabalho foi realizar uma prospecção fitoquímica preliminar, o potencial antimicrobiano e antioxidante dos extratos de E. involucrata. A partir das folhas de E. involucrata, foram obtidos extratos aquosos e orgânicos com os seguintes solventes (etanol, metanol, hexano, acetona, diclorometano e acetato de etila). A prospecção fitoquímica detectou a presença de saponinas, esteroides, flavonóides e taninos nos extratos. Os extratos etanólico e metanólico apresentaram atividade antimicrobiana para a maioria das cepas bacterianas testadas, assim como para a levedura Candida albicans, com concentrações entre 3,12 e 50 mg/mL. Os extratos etanólico e metanólico apresentaram alto potencial de sequestro de radicais livres (> 90%). O extrato metanólico apresentou IC50 estatisticamente igual ao encontrado para o antioxidante comercial BHT (p <0,05). Os extratos brutos obtidos com etanol e metanol foram os mais promissores. Esses resultados sugerem que os extratos metanólico, etanólico e aquoso são uma fonte promissora de bioativos naturais.

Antimicrobial, antioxidant activity and phytochemical prospection of Eugenia involucrata DC. leaf extracts

Abstract The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p 0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.

Resumo A espécie Eugenia involucrata DC. é uma planta nativa do Brasil e tradicionalmente utilizada para problemas intestinais, porém, poucas pesquisas documentam sobre seu potencial biológico e perfil fitoquímico. Assim, o objetivo deste trabalho foi realizar uma prospecção fitoquímica preliminar, o potencial antimicrobiano e antioxidante dos extratos de E. involucrata. A partir das folhas de E. involucrata, foram obtidos extratos aquosos e orgânicos com os seguintes solventes (etanol, metanol, hexano, acetona, diclorometano e acetato de etila). A prospecção fitoquímica detectou a presença de saponinas, esteroides, flavonóides e taninos nos extratos. Os extratos etanólico e metanólico apresentaram atividade antimicrobiana para a maioria das cepas bacterianas testadas, assim como para a levedura Candida albicans, com concentrações entre 3,12 e 50 mg/mL. Os extratos etanólico e metanólico apresentaram alto potencial de sequestro de radicais livres (> 90%). O extrato metanólico apresentou IC50 estatisticamente igual ao encontrado para o antioxidante comercial BHT (p 0,05). Os extratos brutos obtidos com etanol e metanol foram os mais promissores. Esses resultados sugerem que os extratos metanólico, etanólico e aquoso são uma fonte promissora de bioativos naturais.

Antimicrobial, antioxidant activity and phytochemical prospection of Eugenia involucrata DC. leaf extracts / Atividade antimicrobiana, antioxidante e prospecção fitoquímica dos extratos das folhas de Eugenia involucrata DC

Abstract The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p <0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.

Resumo A espécie Eugenia involucrata DC. é uma planta nativa do Brasil e tradicionalmente utilizada para problemas intestinais, porém, poucas pesquisas documentam sobre seu potencial biológico e perfil fitoquímico. Assim, o objetivo deste trabalho foi realizar uma prospecção fitoquímica preliminar, o potencial antimicrobiano e antioxidante dos extratos de E. involucrata. A partir das folhas de E. involucrata, foram obtidos extratos aquosos e orgânicos com os seguintes solventes (etanol, metanol, hexano, acetona, diclorometano e acetato de etila). A prospecção fitoquímica detectou a presença de saponinas, esteroides, flavonóides e taninos nos extratos. Os extratos etanólico e metanólico apresentaram atividade antimicrobiana para a maioria das cepas bacterianas testadas, assim como para a levedura Candida albicans, com concentrações entre 3,12 e 50 mg/mL. Os extratos etanólico e metanólico apresentaram alto potencial de sequestro de radicais livres (> 90%). O extrato metanólico apresentou IC50 estatisticamente igual ao encontrado para o antioxidante comercial BHT (p <0,05). Os extratos brutos obtidos com etanol e metanol foram os mais promissores. Esses resultados sugerem que os extratos metanólico, etanólico e aquoso são uma fonte promissora de bioativos naturais.

Antimicrobial and insecticidal effects of essential oil and plant extracts of Myrcia oblongata DC in pathogenic bacteria and Alphitobius diaperinus / Aplicação de óleo e extratos de Myrcia oblongata DC em bactérias patogênicas e controle biológico de Alphitobius diaperinus

The secondary metabolism products of plants have influenced great economic interest, given their chemical diversity and biological activities. Because of this, this study evaluates the phytochemical composition, antimicrobial activity, insecticidal, and antioxidant activity of plant extracts and oil of Myrcia oblongata. Saponins, steroids, triterpenoids, tannins, and flavonoids were detected. The extracts showed antimicrobial capacity on the tested microorganisms, except for the methanolic extract, which showed no activity for P. mirabilis and S. enteritidis. Regarding the analysis of antioxidant compounds, the hexanic, ethyl acetate and acetone extracts showed higher antioxidant activities and also higher insecticidal performance on Alphitobius diaperinus larvae, resulting in 80% adult mortality. The results reported here show that there may be a relationship between antioxidant potential and the insecticidal effect of Myrcia oblongata DC. The components present in both the extract and the oil can be used as natural alternative to synthetic compounds in the biological control of parasites and pathogenic microorganisms.

Os produtos do metabolismo secundário das plantas têm despertado grande interesse econômico, dada sua diversidade química e atividades biológicas. Neste sentido, o estudo objetivou avaliar a composição fitoquímica, atividade antimicrobiana, inseticida e antioxidante dos extratos vegetais e óleo de Myrcia oblongata. Foram detectados a presença de saponinas, esteróides, triterpenóides, taninos e flavonóides. Os extratos apresentaram capacidade antimicrobiana sobre os microrganismos testados, exceto o extrato metanólico que não demonstrou atividade para P. mirabilis e S. Enteritidis. Quanto a análise de compostos antioxidantes observou-se que os extratos hexânico, acetato de etila e acetona apresentaram maiores atividades antioxidantes e também maior performance inseticida sobre a larva Alphitobius diaperinus e exibindo mortalidade de 80% na fase adulta. Os resultados aqui reportados mostram que pode haver uma relação entre potencial antioxidante e efeito inseticida do óleo de Myrcia oblongata; os componentes presentes tanto no extrato como o oléo podem ser utilizados como alternativa natural aos compostos sintéticos no controle biológico de parasitas e microrganismos patogênicos.

Antimicrobial and insecticidal effects of essential oil and plant extracts of Myrcia oblongata DC in pathogenic bacteria and Alphitobius diaperinus / Aplicação de óleo e extratos de Myrcia oblongata DC em bactérias patogênicas e controle biológico de Alphitobius diaperinus

The secondary metabolism products of plants have influenced great economic interest, given their chemical diversity and biological activities. Because of this, this study evaluates the phytochemical composition, antimicrobial activity, insecticidal, and antioxidant activity of plant extracts and oil of Myrcia oblongata. Saponins, steroids, triterpenoids, tannins, and flavonoids were detected. The extracts showed antimicrobial capacity on the tested microorganisms, except for the methanolic extract, which showed no activity for P. mirabilis and S. enteritidis. Regarding the analysis of antioxidant compounds, the hexanic, ethyl acetate and acetone extracts showed higher antioxidant activities and also higher insecticidal performance on Alphitobius diaperinus larvae, resulting in 80% adult mortality. The results reported here show that there may be a relationship between antioxidant potential and the insecticidal effect of Myrcia oblongata DC. The components present in both the extract and the oil can be used as natural alternative to synthetic compounds in the biological control of parasites and pathogenic microorganisms.

Os produtos do metabolismo secundário das plantas têm despertado grande interesse econômico, dada sua diversidade química e atividades biológicas. Neste sentido, o estudo objetivou avaliar a composição fitoquímica, atividade antimicrobiana, inseticida e antioxidante dos extratos vegetais e óleo de Myrcia oblongata. Foram detectados a presença de saponinas, esteróides, triterpenóides, taninos e flavonóides. Os extratos apresentaram capacidade antimicrobiana sobre os microrganismos testados, exceto o extrato metanólico que não demonstrou atividade para P. mirabilis e S. Enteritidis. Quanto a análise de compostos antioxidantes observou-se que os extratos hexânico, acetato de etila e acetona apresentaram maiores atividades antioxidantes e também maior performance inseticida sobre a larva Alphitobius diaperinus e exibindo mortalidade de 80% na fase adulta. Os resultados aqui reportados mostram que pode haver uma relação entre potencial antioxidante e efeito inseticida do óleo de Myrcia oblongata; os componentes presentes tanto no extrato como o oléo podem ser utilizados como alternativa natural aos compostos sintéticos no controle biológico de parasitas e microrganismos patogênicos.

Antimicrobial and insecticidal effects of essential oil and plant extracts of Myrcia oblongata DC in pathogenic bacteria and Alphitobius diaperinus

Abstract The secondary metabolism products of plants have influenced great economic interest, given their chemical diversity and biological activities. Because of this, this study evaluates the phytochemical composition, antimicrobial activity, insecticidal, and antioxidant activity of plant extracts and oil of Myrcia oblongata. Saponins, steroids, triterpenoids, tannins, and flavonoids were detected. The extracts showed antimicrobial capacity on the tested microorganisms, except for the methanolic extract, which showed no activity for P. mirabilis and S. enteritidis. Regarding the analysis of antioxidant compounds, the hexanic, ethyl acetate and acetone extracts showed higher antioxidant activities and also higher insecticidal performance on Alphitobius diaperinus larvae, resulting in 80% adult mortality. The results reported here show that there may be a relationship between antioxidant potential and the insecticidal effect of Myrcia oblongata DC. The components present in both the extract and the oil can be used as natural alternative to synthetic compounds in the biological control of parasites and pathogenic microorganisms.

Resumo Os produtos do metabolismo secundário das plantas têm despertado grande interesse econômico, dada sua diversidade química e atividades biológicas. Neste sentido, o estudo objetivou avaliar a composição fitoquímica, atividade antimicrobiana, inseticida e antioxidante dos extratos vegetais e óleo de Myrcia oblongata. Foram detectados a presença de saponinas, esteróides, triterpenóides, taninos e flavonóides. Os extratos apresentaram capacidade antimicrobiana sobre os microrganismos testados, exceto o extrato metanólico que não demonstrou atividade para P. mirabilis e S. Enteritidis. Quanto a análise de compostos antioxidantes observou-se que os extratos hexânico, acetato de etila e acetona apresentaram maiores atividades antioxidantes e também maior performance inseticida sobre a larva Alphitobius diaperinus e exibindo mortalidade de 80% na fase adulta. Os resultados aqui reportados mostram que pode haver uma relação entre potencial antioxidante e efeito inseticida do óleo de Myrcia oblongata; os componentes presentes tanto no extrato como o oléo podem ser utilizados como alternativa natural aos compostos sintéticos no controle biológico de parasitas e microrganismos patogênicos.

Miositis osificante traumática en recién nacido: reporte de caso / Traumatic myositis ossificans in newborn: a case report

La miositis osificante traumática (MOT) es una enfermedad en la que ocurre osificación heterotópica en dos a cuatro semanas tras uno o múltiples traumatismos. El objetivo de este artículo es describir las características clínicas y radiológicas de un caso de MOT en un recién nacido (RN) después de la canulación intravenosa de vía periférica, poco frecuente en la práctica clínica en neonatología. Presentamos a un RN pretérmino de 33 semanas en que, a los 20 días de vida, se evidenció lesión tumoral en el tercio distal del antebrazo izquierdo de 3 cm por 2 cm, que no impresionaba dolor, ni limitación a la movilización, y en la que no había signos infecciosos. El resto del examen físico osteomuscular era normal. En la zona de lesión, tres semanas antes, se había instalado un catéter intravenoso periférico (CIVP). Una radiografía del antebrazo izquierdo demostró lesión calcificada al nivel de las partes blandas, sin disrupción de las estructuras óseas adyacentes; la ecografía del antebrazo reveló una imagen focal ovalada, de contornos parcialmente definidos, con sombra acústica posterior; el resto de los estudios de huesos largos era normal. Los niveles séricos de fosfatasa alcalina, calcio, fósforo también eran normales. En vista de la lesión tumoral al examen físico y la imagen calcificada en partes blandas a través de radiografía simple, con antecedente de microtraumas de VVP, se concluyó MOT. Se hizo seguimiento, con disminución del tamaño hasta que la lesión desapareció a los cuatro meses. No requirió control radiológico. La MOT es infrecuente en el RN, y, en general, la resolución es autolimitada y tiene buen pronóstico

Traumatic myositis ossificans (TMO) is a disorder in which heterotopic ossification occurs two to four weeks after one or multiple traumas. The goal of the present article is to describe the clinical and radiological characteristics of a case of TMO in a newborn (NB) after a peripheral intravenous cannulation, a rare procedure in the clinical practice of neonatology. The patient is a premature 33-week-old NB who, 20 days after birth, presented with a 3 cm x 2 cm lump in the distal third of the left forearm that did not seem to cause pain or to limit movements, and with no evidence of infection. The rest of the physical exam was within normal limits. Three weeks before the lesion, a peripheral intravenous catheter (PIVC) was placed in that area. A radiograph of the left forearm showed soft-tissue calcification without disruption of adjacent bone structures. Ultrasound revealed a focal, oval soft tissue lesion with partially-defined borders and posterior acoustic shadow; the rest of study showed normal long bones. The serum levels of alkaline phosphatase, calcium, and phosphorus were all normal. In view of the tumor lesion on the physical examination and the calcified image in softtissue on plain X-ray and a recent history of PIVC microtrauma, we reached to the diagnoses of TMO. During the follow-up, the lesion decreased in size until it completely disappeared four months after the diagnosis. No radiological control was needed. Uncommon in NBs, TMO is generally self-limited and with a good prognosis

Short-term exposure of mice to cigarette smoke and/or residual oil fly ash produces proximal airspace enlargements and airway epithelium remodeling

Chronic obstructive pulmonary disease (COPD) is associated with inflammatory cell reactions, tissue destruction and lung remodeling. Many signaling pathways for these phenomena are still to be identified. We developed a mouse model of COPD to evaluate some pathophysiological mechanisms acting during the initial stage of the disease. Forty-seven 6- to 8-week-old female C57/BL6 mice (approximately 22 g) were exposed for 2 months to cigarette smoke and/or residual oil fly ash (ROFA), a concentrate of air pollution. We measured lung mechanics, airspace enlargement, airway wall thickness, epithelial cell profile, elastic and collagen fiber deposition, and by immunohistochemistry transforming growth factor-β1 (TGF-β1), macrophage elastase (MMP12), neutrophils and macrophages. We observed regional airspace enlargements near terminal bronchioles associated with the exposure to smoke or ROFA. There were also increases in airway resistance and thickening of airway walls in animals exposed to smoke. In the epithelium, we noted a decrease in the ciliated cell area of animals exposed to smoke and an increase in the total cell area associated with exposure to both smoke and ROFA. There was also an increase in the expression of TGF-β1 both in the airways and parenchyma of animals exposed to smoke. However, we could not detect inflammatory cell recruitment, increases in MMP12 or elastic and collagen fiber deposition. After 2 months of exposure to cigarette smoke and/or ROFA, mice developed regional airspace enlargements and airway epithelium remodeling, although no inflammation or increases in fiber deposition were detected. Some of these phenomena may have been mediated by TGF-β1.

Lisina digestível para frangos de corte machos entre 12 e 22 dias de idade / Digestible lysine for male broilers from 12 to 22 days of age

Avaliou-se nível de lisina digestível para 1050 frangos de corte dos 12 aos 22 dias de idade. O delineamento experimental foi inteiramente ao acaso, com cinco tratamentos, sete repetições e 30 aves por unidade experimental. Os tratamentos foram: 1,05; 1,10; 1,15; 1,20 e 1,25 por cento de lisina digestível. Avaliaram-se ganho de peso, consumo de ração, conversão alimentar, composição e deposição de nutrientes corporais. Foram constatados efeitos quadráticos de lisina digestível no consumo de ração e resposta linear ascendente no peso da carcaça. Na composição química da carcaça, houve resposta quadrática do nível de lisina na concentração de proteína. As taxas de deposição proteica, deposição de água, da carcaça e do corpo total tiveram aumento linear em resposta ao acréscimo de lisina na dieta. O aumento da concentração de lisina, todavia, coincidiu com a redução da matéria mineral nas vísceras e sangue e no corpo total. Considerado o desempenho, o nível 1,1 por cento de lisina digestível atendeu às necessidades do frango de corte entre o 12º e o 22º dia de idade. Consideradas a composição química e as taxas de deposição dos nutrientes corporais, a demanda pelo aminoácido digestível torna-se igual ou maior que 1,25 por cento.

The lysine level was evaluated for 1,050 male broilers from 12 to 22 days of age. The experimental design was completely randomized with five treatments, seven replications, and 30 birds per experimental unit. The treatments were: 1.05, 1.10, 1.15, 1.20, and 1.25 percent of digestible lysine. Weight gain, feed intake, feed: gain ratio, body composition, and nutrient deposition were measured. There were quadratic effects of digestible lysine on feed intake and an ascendant linear effect on carcass weight. In the carcass chemical composition, there was a quadratic response of lysine level in protein concentration. The deposition rates in carcass protein, carcass water, and whole body had a linear increase as response to lysine addition to diet. The lysine level increase however coincided with the mineral decrease on blood, offal, and whole body. Basing on performance, the digestible lysine 1.1 percent level attended the broiler needs from 12 to 22 days of age. On the other hand, considering the chemical composition and the nutrient accretion rates, the need for digestible amino acid was equal or higher than 1.25 percent.

Cirrosis hepática: medidas preventivas de algunas de sus complicaciones / Liver cirrhosis: some complications preventive measures

Cirrosis hepática es una enfermedad prevalente en el mundo. Su diagnóstico y tratamiento debe ser dominio de todo médico general e internista. En los últimos años han ocurrido notables avances en el diagnóstico, fisiopatología, tratamiento y en la identificación de factores pronósticos de cirrosis y de sus complicaciones mayores. Particular atención ha existido en desarrollar medidas preventivas de sus complicaciones como por ejemplo: hemorragia por várices esofágicas, peritonitis bacteriana espontánea, síndrome hepatorrenal. Estas medidas preventivas han influido de forma muy favorable en el pronóstico de los pacientes cirróticos. En este artículo se discuten las diversas estrategias preventivas que han demostrado su efectividad en cirrosis hepática

Liver cirrhosis is a prevalent disease around the world. Its diagnosis and treatment must be in the knowledge of every general physicians and internist.Over the last years, there have been notorious advances in the diagnosis, pathophysiology, treatment and the identification of prognosis factors for cirrhosis major complications.There has been particular attention to the development of preventive measures for its complications, such as variceal bleeding, spontaneous bacterial peritonitis, hepatorenal syndrome. These preventive measures have had a favorable influence on the prognosis of cirrhotic patients. This article discusses several preventive strategies that have proven its effectiveness in hepatic cirrhosis

Prevalencia de la resistencia a metrodinazol, claritromicina y tetraciclina en Helicobacter pylori aislado de pacientes de la Región Metropolitana / Prevalence of metronidazole, clarithromycin and tetracycline resistance in Helicobacter pylori isolated from Chilean patients

Background: Helicobacter pylori is a pathogenic bacterium that infects a significant number of individuals. At present, therapeutic strategies to eradicate this bacterium depend on our knowledge of its resistance to antimicrobials. Aims: To evaluate the primary resistance of H pylori to metronidazole (Mtz), clarithromycin (Cla), and tetracycline (Tet) in symptomatic out-patients. Material and Methods: Fifty independent isolates of H pylori were obtained by endoscopy-assisted gastric biopsy from patients attending the University of Chile Clinical Hospital, that previously had not been treated with an eradication regime against this bacterium. The minimal inhibitory concentration of each antimicrobial was determined by agar dilution method. Results: Forty five and 27 percent of the isolates were found to be resistant to Mtz and Tet, respectively; the majority of these resistant isolates were from patients older than 21 years. Twenty percent of isolates were resistant to Cla; these were distributed evenly among different ages. Thirty two percent of the isolates were resistant to two or more of the antimicrobials. Conclusions: The high frequency of naturally occurring, antimicrobial-resistant strains of H pylori poses a national and world-wide problem for public health.

Prevalencia de la infección por helicobacter pylori según ensayo de la ureasa en pacientes derivados a la Unidad de Endoscopía del Hospital Clínico de la Universidad de Chile / Prevalence of infection by helicobacter pylori by urease test in patients derivates to the endoscopic section of Hospital Clínico de la Universidad de Chile

Helicobacter pylori is a pathogenic bacterium that infects a significant number of individuals. In Chile about 79 percent of the population is colonized. Aims: This study evaluate the prevalence of the H. pylori infection in symptomatic outpatients. Materials and Methods: 276 non selected patients were enrolled from Endoscopic Unit of Clinical Hospital of the University of Chile. The bacterium was detected by urease test. Results: H. pylori infection was found in 44,9 percent patients. Infection was higher in younger patients, 53,8 percent between 21-60 years, and was lower in older patients, 25,6 percent in older than 60 years. The risk of being H. pylori carrier is twofold higher in persons younger than 60 years as compared to those older than 60 years. Conclusion: The age would be modifier factors for H. pylori infection risk.

Seguimientos de anticuerpos contra hepatitis A y E en una cohorte de niños de bajo nivel socioeconómico / Hepatitis A and E virus antibodies in Chilean children of low socioeconomic status. A one year follow-up study

Background: The seroprevalence of antibodies against hepatitis A virus (HAV) is decreasing in many Latin American countries, along with improvements in sanitary standards. However, there is no information available about low socioeconomic status (LSE) populations. Aim: To assess the evolution of hepatitis A and E virus antibodies in a cohort of LSE Chilean children. Material and methods: One hundred sixty eight children aged four years, 97 males, coming from public primary care clinics, were studied. Two blood samples were obtained with an interval of one year. Anti-HAV and anti-hepatitis E virus (HEV) antibodies, were detected by ELISA using Abbott kits. Results: Anti-HAV was positive in 19 children (11.3%). After one year of follow-up, only 10 children had sustained reactivity (52.6%). Fourteen children, initially negative, became positive during the follow up (9.4%). Antibody titers to HAV were significantly higher in samples that remained positive, compared with those that lost reactivity. Anti-HEV was found positive in two children (1.2%). One remained positive and the other became negative. Conclusions: In this cohort of LSE Chilean children, the prevalence to antibodies against HAV and HEV is low. Follow-up detected loss of reactivity to HAV in nearly one half of the children, probably related to lower antibody levels.

Efeitos agudos da aplicacao do BiPAP sobre a tolerancia ao exercicio fisico em pacientes com doenca pulmonar obstrutiva cronica (DPOC) / Acute effects of BiPAP application on physical exercise tolerance among chronic obstructive pulmonary disease patients

Pacientes com Doenca Pulmonar Obstrutiva Cronica (DPOC) apresentam prejuizos sobre a capacidade de exercicio fisico. A ventilacao nao-invasiva (VNI) tem sido associada ao exercicio fisico com o objetivo de melhorar a tolerancia aos esforcos nesses pacientes. Objetivos: avaliar os efeitos agudos da aplicacao da pressao positiva nas vias aereas por dois niveis pressoricos(BiPAP) sobre a tolerancia ao exercicio fisico, a dispneia e a saturacao periferica de oxigenio (SpO2) em pacientes com DPOC. Material e metodos: foram estudados 27 pacientes com DPOC (68+-8,3 anos) com volume expiratorio forcado no 1 segundo (VEEF1) < 50 por cento do previsto e sintomas de dispneia aos esforcos. os pacientes foram submetyidos, de modo randomizado, a dois testes de caminhada de 6 minutos em esteira (TC6E); um com a aplicacao do BiPAP, com niveis pressoricos inspirados de 14+-1 cmH2O, e o outro sem o BiPAP

Niveles de Depresión y Sintomatología característica en pacientes adultos con Diabetes Mellitus tipo 2

Las personas que padecen diabetes son dos veces más propensas a sufrir depresión, por lo cual es de gran importancia conocer la comorbilidad entre la depresión, la diabetes y el impacto en la calidad de vida del individuo afectado. El objetivo del presente estudio fue determinar los niveles de depresión y su sintomatología característica en pacientes adultos con diabetes mellitus tipo 2. Para ello fueron estudiados 40 pacientes diabéticos con edades comprendidas entre los 30 y los 65 años de edad que acudieron por primera vez a la consulta de diabetes en el Centro de Investigaciones Endocrino - Metabólicas "Dr. Felix Gómez", utilizando el inventario de depresión de Beck que es un instrumento de evaluación psicológica conformado por 21 items que mide diferentes síntomas cognitivos, afectivos, somáticos e interpersonales de la depresión. Los resultados indican que un 55% de los pacientes evidenciaban algún nivel de depresión, al comparar por sexo se determinó que los sujetos de sexo femenino evidenciaron niveles más graves de depresión (1,4 ± 0,2 los femeninos vs. 0,4 ± 0,1 los masculinos; p < 0,02) y un mayor índice de sintomatología característica de esta alteración del estado de ánimo que los hombres. Las conclusiones del presente estudio confirman la comorbilidad entre la depresión y la Diabetes Mellitus tipo 2 y plantean la necesidad de la atención psicológica a poblaciones clínicas con esta condición crónica debido al impacto potencial que podría tener la depresión en el manejo y la calidad de vida de pacientes con Diabetes.

Adults with Diabetes are twice more likely to suffer depression, and for this reason is important to know the relationship between depression, diabetes and how this affects an individuals quality of life. The aim of this study was to determine in adult type 2 diabetic patients, depression levels and its main symptoms. 40 type 2 diabetic patients, between 30 and 65 years of age, who assisted for the first time to the Endocrine - Metabolic Research Center "Dr. Félix Gómez", were studied. Depression levels were quantified using the Beck Depression Inventory. Results show that 55% of these patients had some level of depression. Female patients had higher levels of depressión than male patients (female 1,4 ± 0,2 vs. 0,4 ± male 0,1; p < 0,02) and more severe depressive symptoms than men. This study confirms the comorbility between depression and type 2 diabetes mellitus and shows the necesity of psycological treatment.

Resistencia antimicrobiana en Helicobacter pylori: aspectos clínicos y moleculares / Antimicrobial resistance of Helicobacter pylori: clinical and molecular aspects

Helicobacter pylori is a relevant pathogen for gastroduodenal diseases in human beings. Although its eradication often improves gastroduodenal diseases, H pylori is acquiring an elevated rate of resistance to various antimicrobials, such as metronidazole, clarithromycin, tetracycline and amoxicillin. Multi-drug resistance is a major problem to select the appropriate treatment of infectious diseases. To improve our understanding on the com-plexity of the problem, in this article we review the resistance mechanisms and give an update on H pylori antimicrobial resistance (Rev Méd Chile 2003; 131: 1313-20).