ABSTRACT
A morfologia das células epiteliais do intestino médio de larvas de Anticarsia gemmatalis (Hübner) é descrita em microscopia de luz e eletrônica de transmissão. O intestino médio de A. gemmatalis é a maior porção do trato digestivo, apresentando três regiões distintas: proximal, média e distal. Sua parede é formada por um tecido epitelial pseudo-estratificado colunar, com quatro tipos celulares: células colunares, caliciformes, regenerativas e endócrinas. As células colunares, observadas em maior quantidade, são altas, apresentam longas e numerosas microvilosidades na porção apical e na porção basal as invaginações da membrana formam o labirinto basal. As células caliciformes apresentam uma grande câmara central delimitada por projeções citoplasmáticas repletas de mitocôndrias no interior. As células regenerativas têm o citoplasma mais elétron-denso e com poucas organelas. As células endócrinas são caracterizadas pela presença de grânulos secretores bastante elétron-densos concentrados principalmente no citoplasma basal da célula.
Subject(s)
Animals , Epithelial Cells , Intestines , Lepidoptera , Larva/ultrastructure , Microscopy, ElectronABSTRACT
We studied the ultrastructural aspects of pre-pupae and pupae ovaries of Dermatobia hominis. Physiological degeneration of gonial cells was observed: (a) after the ovarioles differentiation, in the oogonia residing in the apical region of the ovary; (b) at the beginning of vitellogenesis, in the cystoblasts close to the terminal filament. The significance of gonial cell degeneration was correlated with the physiological changes wich occur in the ovary during development
Subject(s)
Animals , Diptera , Ovary/ultrastructureABSTRACT
No periodo compreendido entre abril e outubro de 1988, foram estudadas 481 gestantes de primeira consulta, as quais estavam inscritas no Programa de Atendimento a Gestante em oito Centros de Saude da Secretaria do Estado da Saude de Sao Paulo. Do total estudado, 86 gestantes nao trouxeram amostras de fezes para analise, mesmo apos varias solicitacoes. A idade media da populacao estudada foi de 24,5 anos (idade minima de 14 e maxima de 46 anos); a renda media das familias das gestantes foi de 0,97 SMPC (salario minimo per capita) e o numero medio de pessoas que compunham suas familias foi de quatro (um a quinze pessoas). A prevalencia de verminose foi de 45,1 por cento (n=395). Os parasitas mais frequentes foram: Ascaris lumbricoides (19,0 por cento); ancilostomideos (16,7 por cento) e Trichuris trichiura (15,9 por cento). Das 248 gestantes infectadas de enteroparasitas, 70 (28,2 por cento) eram portadoras somente dos seguintes protozoarios: Endolimax nana, Entamoeba coli e Iodamoeba butschllii. A prevalencia de parasitas intestinais foi significativamente maior (p<0,05) nas gestantes pertencentes as familias com renda de ate 0,5 SMPC e compostas por 5 pessoas ou mais.
Subject(s)
Pregnancy , Adolescent , Adult , Middle Aged , Humans , Female , Ambulatory Care , Helminthiasis/epidemiology , Pregnancy Complications, Infectious , Protozoan Infections/epidemiology , Brazil/epidemiology , Eukaryota/isolation & purification , Helminthiasis/parasitology , Helminths/isolation & purification , Prevalence , Protozoan Infections/parasitology , Socioeconomic FactorsABSTRACT
The ultrastructure and distribution of gonial and somatic cells in the ovary of Dermatobia hominis was studied during the 3rd larval instar. In larvae weighing between 400 and 500 mg, the ovary is partially divided into basal and apical regions by oblong somatic cells that penetrate from the periphery; these cells show ovoid nucleus and cytoplasm full of microtubules. In both regions, gonial cells with regular outlines, large nucleus and low electron-density cytoplasm are scattered among the interstitial somatic cells. These later cells have small nucleus and electrodense cytoplasm. Clear somatic cells with small nucleus and cytoplasm of very low electron-density are restrict to the apical region of the gonad. Degenerating interstitial somatic cells are seen in the basal portion close to the ovary peduncle. During all this larval period the morphological features of the ovary remain almost the same. At the end of the period there is a gradual deposition of glycogen in the cytoplasm of the somatic cells, increase in the number and density of their mitochondria plus nuclear modification as membrane wrinkling and chromatin condensation in masses
Subject(s)
Animals , Female , Diptera/ultrastructure , Ovary/ultrastructure , Brazil , Diptera/growth & development , Larva/ultrastructureABSTRACT
Ovaries up to the 8th day pupae of Dermatobia hominis were studied by transmission electron microscopy. Ovarioles were recognized in ovaries of 4-day old pre-pupae, surrounded by a thin tunica propria of acellular fibrilar material similar in structure to the internal portion of the external tunica of the ovary. There is continuity of the tunica propria and the ovarian tunica, indicating that the former structure originates from the tunica externa. In 5 to 7-day pupae the interstitial somatic cells from the apical region of the ovary, close to the ovarioles, show delicate filamentous material inside of their rough endoplasmic reticulum cisternae; similar material is seem among these cells. Our observations suggest that interstitial somatic cells do not originate the tunica propria but contribute to its final composition