ABSTRACT
Mycobaterium leprae infection was investigated in armadillos from the State of Espírito Santo, Brazil. The ML Flow test was performed on 37 nine-banded armadillos and positive results were found in 11 (29.7 percent). The ML Flow test may be used to identify possible sources of Mycobaterium leprae among wild armadillos.
Tem sido pesquisado infecção pelo Mycobaterium leprae em tatus provenientes do estado do Espírito Santo-Brasil. O teste rápido ML Flow, foi realizado em 37 tatus selvagens, tendo sido positivo em 11 (29,7 por cento). O teste de ML Flow pode ser utilizado para identificar possíveis fontes de Mycobaterium leprae em tatus selvagens.
Subject(s)
Animals , Antigens, Bacterial/blood , Armadillos/microbiology , Glycolipids/blood , Immunoassay/methods , Immunoglobulin M/blood , Leprosy/veterinary , Mycobacterium leprae/immunology , Brazil , Leprosy/diagnosis , Sensitivity and SpecificitySubject(s)
Humans , Leprosy/classification , Leprosy/diagnosis , Leprosy/therapy , Mycobacterium lepraeABSTRACT
A síndrome de Chediaki-Higashi (SCH) é distúrbio raro, de caráter autossômico recessivo, caracterizada poralbinismo parcial e imunodeficiência celular com presença de grânulos gigantes nos leucócitos e outras células. Os autores apresentam um caso típico, com revisäo da literatura sobre etiologia, patogenia, evoluçäo, diagnóstico clínico laboratorial, diagnóstico diferencial e tratamento
Subject(s)
Humans , Female , Infant , Chediak-Higashi Syndrome/diagnosis , Chediak-Higashi Syndrome/etiology , Chediak-Higashi Syndrome/drug therapy , Bone Marrow Transplantation , Diagnosis, DifferentialABSTRACT
The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5 percent gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals