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RBCF, Rev. bras. ciênc. farm. (Impr.) ; 42(2): 245-249, abr.-jun. 2006. tab
Article in Portuguese | LILACS | ID: lil-454546

ABSTRACT

This communication describes a method for adsorbing the invertase (EC.3.2.1.26) on DOWEX'registred mark' anion exchange resin. Among the types of DOWEX'registred mark' resins studied (1x8:50-400; 1x4:50-400 and 1x2:100-400), 1X4-200 was the most suitable, because it adsorbed the invertase molecules completely and the complex 1X4-200/invertase retained 100% of the catalytic activity. Moreover, no leakage of enzyme from the support was noted at the end of the sucrose hydrolysis


O presente trabalho descreve um método de adsorção da invertase (EC. 3.2.1.26) na resina de troca aniônica do tipo Dowex®. Entre os tipos de resinas Dowex® estudados (1x8:50-400; 1x4:50-400 e 1x2:100-400), 1x4-200 foi a mais apropriada devido à completa adsorção das moléculas de invertase e a sua retenção de atividade catalítica de 100% do complexo 1x4-200/invertase. Salienta-se ainda a ausência do desprendimento da enzima do suporte após o término da hidrólise da sacarose


Subject(s)
Enzymes, Immobilized/metabolism , Adsorption , Hydrolysis
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