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1.
Saudi Medical Journal. 2006; 27 (2): 215-218
in English | IMEMR | ID: emr-80687

ABSTRACT

To evaluate the relationship between vaginal douching and vaginal infections among women in early pregnancy. We conducted this study in the Department of Gynecology and Obstetrics, Family Planning Center, Dr. E. Hayri Ustundag Hospital, Izmir, Turkey, between March 2003 and December 2004. We examined the vaginal swabs of 129 women, asking for termination of pregnancy in a family-planning center as both wet-preparations and cultures for vaginal microorganisms, and recorded the informations on women's vaginal douching habit. Among 129 women examined, 80 had at least one type of vaginal microorganisms. Of 67 vaginal douche users, 48 [71.6%] had at least one type of vaginal organisms, whereas of 62 nonusers, only 32 [51.6%] had microorganism, although age, educational status, coital frequency, age at the first intercourse were not statistically different between the vaginal douche-users and non-users. Especially, Group B Streptococcus [GBS], Enterococcus spp. and Candida spp. were found more frequent in vaginal douche-user women. We found that vaginal douching tends pregnant women to genital tract the incidence of vaginal infections, especially those caused by Enterococcus spp. and GBS. As such infections may render such women to high risk in terms of perinatal mortality and morbidity, it would be appropriate to discourage vaginal douching in pregnant women


Subject(s)
Humans , Female , Vaginal Diseases/therapy , Vaginal Diseases/adverse effects , Pregnancy , Pregnancy Complications, Infectious
2.
Saudi Medical Journal. 2005; 26 (12): 1911-1913
in English | IMEMR | ID: emr-74762

ABSTRACT

To examine the survival of Echinococcus multilocularis [E. multilocularis] and the formation of protoscoleces, under refrigerated conditions over a 43-day observation period. We conducted this study in Ege University, School of Medicine, from May-December, 2004. We included 4 healthy females and 2 males, 8-12 week old M. unguiculatus species [approximately 60-70 gr] for the study, as they are known to be susceptible to E. multilocularis. In this experimental study, we aimed to define a technique to keep the strains during these passages. We found preserved viability and virulence of E. multilocularis stored in RPMI 1640 plus 10% fetal calf serum at +40C. We accomplished the infection of the Meriones unguiculatus after in-vivo passaging from that flask, which we kept at +40C, and this proves the viability of protoscoleces and membranous structures perfectly at the 43rd day. We also found that E. multilocularis metacestodes and the formation of protoscoleces keep their viability up to 60 days and virulence up to 43 days under this condition. We recommend storing an extra flask during each passage as a precaution against losing strains


Subject(s)
Male , Female , Animals, Laboratory , Echinococcus/classification , Echinococcus/growth & development , Echinococcus/parasitology , Mice
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