ABSTRACT
To determine the genetic diversity of Haloxylon ammodendron collected from 14 sites in 5 provinces, 103 H. ammodendron samples of 12 wild populations and 2 cultivated which collected from 14 sites in 5 provinces were analyzed by amplified fragment length polymorphism (AFLP) DNA markers. PopGen32 and NTSYSpc2.1 was applied to evaluate genetic diversity of H. ammodendron populations. The average percentage of polymorphic loci (PPL) of total H. ammodendron populations was 94.13%, the average Nei's gene diversity index (H(e)) from 14 populations was 0.308 0, and the Shannon's genetic diversity index (I) was 0.467 6. The results indicated that the genetic diversity of H. ammodendron populations was high. Genetic differentiation index (G(st)) was 0.313 8, and the gene flow (N(m)) was 1.093 5 at the population level. The level of gene flow of H. ammodendron showed it possessed the feature of wind-pollinated outcrossing plants. AMOVA analysis indicated that genetic variation of H. ammodendron was much higher within groups (89.34%) than that among groups (10.66%), moreover genetic variation within groups mainly occurred among populations in different producing areas (84.80%). Cluster analysis (UPGMA) was applied to generate dendrogram based on Nei's genetic distances of 14 populations. Samples from Xinjiang and Qinghai were clustered respectively as a clade for their distant genetic relationship, while Samples from Gansu, Inner Mongolia and Ningxia were clustered together for their close genetic relationship. Genetic diversity of H. ammodendron populations is high in China, and genetic differentiation among regions is small, thus abundance within this specie is high at this stage. Therefore, wild nursery and artificial cultivating in different areas are effective measures for the conservation and sustainable utilization of H. ammodendron resources.
Subject(s)
Amaranthaceae , Genetics , Amplified Fragment Length Polymorphism Analysis , China , Evolution, Molecular , Genetic Variation , PhylogenyABSTRACT
Cistanche Herba is one of precious traditional Chinese medicine, which original wild plant resources dropped sharply in recent years. It is urgent to make sustainable utilization. The genus of Cistanche is a total parasitic plant, its physiological ecology and nutrition transfer are very particular. The status of the studies on habitat characteristics, parasitic mechanism and nutrient transport of Cistanche was reviewed, prospect was also given. It can provide reference for the further basic and applied studies on the nutrition transfer, germplasm quality and agriculture practice.
Subject(s)
Agriculture , Biological Transport , Cistanche , Metabolism , Parasitology , Ecosystem , Medicine, Chinese TraditionalABSTRACT
<p><b>OBJECTIVE</b>To study the reproduction characteristic of Cistanche deserticola.</p><p><b>METHOD</b>The reproduction characteristic and growing dynamic in Ningxia plantation were investigated, the contents of boschnaloside and echinacoside were determined during reproduction period.</p><p><b>RESULT</b>The germination period of C. deserticola was in the first ten days of the month, the duration from the germination to flowering lasted about one month. The contents of boschnaloside and echinacoside decreased during reproduction period.</p><p><b>CONCLUSION</b>The reproduction period of C. deserticolais lasts about two months, germination period of C. deserticola is the suitable harvest time.</p>
Subject(s)
Cistanche , Metabolism , Flowers , Metabolism , Germination , Physiology , Glycosides , Metabolism , Plants, Medicinal , Metabolism , Reproduction , Physiology , Seasons , Seedlings , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the kinds and occurrence of pests of Cistanche deserticola and its hosts so as to find ways of pest control.</p><p><b>METHOD</b>Pests of C. deserticola and its hosts were investigated in Xinjiang, Inner Mongolia and Ningxia where C. deserticola grows, the occurrence of Anomala titanis Reitter was investigated in the field, and phoxim was used for field control.</p><p><b>RESULT</b>There are 17 kinds of pests which harm C. deserticola and its hosts. Phoxim 40% EC 1000 times, 2000 times and Phoxim 3% GR [8 kg x (667 m2)(-1)] had 100% controlling effect, and 3% GR [4 kg x (667 m)(-2-1)] had 88.23% controlling effect.</p><p><b>CONCLUSION</b>Many kinds of pests can harm the C. deserticola and its hosts. Phoxim is good for killing A. titanis Reitter.</p>
Subject(s)
Animals , Chenopodiaceae , Cistanche , Coleoptera , Insect Control , Insecticides , Organothiophosphorus Compounds , Plants, MedicinalABSTRACT
<p><b>OBJECTIVE</b>To study the Pollination Characteristic of C. deserticola.</p><p><b>METHOD</b>Four pollination methods were used, cross pollination coming different plant, cross pollination in one plant, wind pollination and no-pollination.</p><p><b>RESULT</b>Cross pollinations coming different plant are fructiferous. The average fructication rate of cross pollination coming different plant is 94.1%; the average fructication rate of cross pollination in one plant is 52.2%; The seed qulitity of cross pollination coming different plant is better than the cross pollination in one plant.</p><p><b>CONCLUSION</b>Cross pollinations coming different plant have a high quantity and quality of seeds of C. deserticola.</p>