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1.
Braz. j. microbiol ; 45(3): 1105-1112, July-Sept. 2014. graf, tab
Article in English | LILACS | ID: lil-727045

ABSTRACT

Studies were conducted to determine the effect of osmotic and matric stress on germination and growth of two Fusarium solani strains, the etiological agent responsible of peanut brown root rot. Both strains had similar osmotic and matric potential ranges that allowed growth, being the latter one narrower. F. solani showed the ability to grow down to -14 MPa at 25 °C in non-ionic modified osmotic medium, while under matric stress this was limited to -8.4 MPa at 25 °C. However, both strains were seen to respond differently to decreasing osmotic and matric potentials, during early stages of germination. One strain (RC 338) showed to be more sensitive to matric than osmotic (non ionic) and the other one (RC 386) showed to be more sensitive to osmotic than matric imposed water stress. After 24 h of incubation, both isolates behaved similarly. The minimum water potential for germination was -8.4 MPa on glycerol amended media and -5.6 MPa for NaCl and PEG amended media, respectively. The knowledge of the water potential range which allow mycelia growth and spore germination of F. solani provides an inside to the likely behaviour of this devastating soilborne plant pathogen in nature and has important practical implications.


Subject(s)
Fusarium/growth & development , Osmotic Pressure , Water/metabolism , Arachis/microbiology , Fusarium/drug effects , Fusarium/radiation effects , Glycerol/metabolism , Plant Diseases/microbiology , Polyethylene Glycols/metabolism , Soil Microbiology , Sodium Chloride/metabolism , Temperature
2.
Medicina (B.Aires) ; 54(3): 221-9, 1994. tab, graf
Article in Spanish | LILACS | ID: lil-141785

ABSTRACT

Se analizan los efectos producidos en el hígado por la isquemia fría en solución eurocollins (EC). Para ello se evalúa la función de cortes provenientes de hígados preservados en EC. Se utilizaron hígados de ratas Wistar, perfundidos con Krebs-Henseleit (KH) luego con EC y conservados a 4º en la misma durante 7, 24 y 48 hs. Finalizados los períodos de preservación, se hicieron cortes, los que fueron incubados 1 hora a 37ºC en KH y se determinaron los parámetros: distribución del agua y electrolitos tisulares, liberación de LDH al medio de incubación, producción de sustancias Ac. Tiobarbitúrico reactivas (SATBR) y Ureagénesis. Los cortes provenientes de hígados preservados desde 7hs, mostraron un incremento del agua tisular, expresado en la expansión del espacio extracelular. También se observaron, una disminución progresiva de la conc. tisular de K+, aumentos de SATBR de la liberación de LDH con el incremento del tiempo de presevación. No se observaron cambios en la ureogénesis. Estos resultados indican que la isquemia fría en EC, provoca cambios en la permeabilidad de membranas, fenómeno que afecta severamente el mecanismos de regulación del volumen tisular y compromete la viabilidad funcional del órgano a transplantar


Subject(s)
Rats , Hypertonic Solutions , Hypothermia, Induced , Liver , Organ Preservation , Biopsy , Liver/metabolism , Liver/pathology , Rats, Wistar
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