T cell receptor excision circles as a tool for evaluating thymic function in young children

The thymus is a primary lymphoid organ responsible for the maturation of T cells as well as the immunological central tolerance. It is in the antenatal period and infancy that it plays its major role. In clinical practice, T cell receptor excision circles (TRECs) are considered a direct and reliable measure of the thymic function. TRECs are a by-product of DNA formation in gene rearrangement of T cell receptors. They are stable and they do not duplicate during mitosis, representing the recent emigrant T cells from the thymus. Despite their importance, TRECs have been neglected by physicians and there is a lack of data regarding thymic function during infancy of healthy children. In order to evaluate thymic function in the first years of life, we propose measuring TRECs as a valuable tool. One hundred and three blood samples from children and adolescents between 3 months and 20 years of age were analyzed. The mean TRECs count was 136.77±96.7 copies of TRECs/μL of DNA. The individuals between 0 and 5 years of age had significantly higher TRECs values than those between 10 and 20 years of age. No significant difference was observed in TRECs values among age groups below 5 years of age. An inverse correlation between TRECs and age was found (r=0.3 P=0.003). These data highlight and validate the evidence of decreased thymus function with age, even during infancy. Awareness should be raised with this important albeit ignored organ.

Mammary botryomycosis: two concomitant cases in mares: case report / Botriomicose mamária: dois casos concomitantes em éguas: relato de caso

Mammary disorders in mares are rare and usually only one animal per paddock is affected. In this report, two mares with 7 and 9 years old, were concomitantly diagnosis of chronic pyogranulomatous mastitis, associated with the Splendore-Hoeppli reaction, indicative of botryomycosis a rare, chronic suppurative disease with microabscess formation, characterized by in vivo formation of eosinophilic materials around microorganisms or biologically inert material. Various bacteria can cause botryomycosis in horses, and the most frequently isolated one is Staphylococcussp., particularly S. aureus. This report confirms the role of Staphylococcus sp.; however, specifically S. hyicus and S. cohnii that prior to the current report, had not been associated with cases of botryomycosis.(AU)

Enfermidades mamárias em éguas são raras e, normalmente, apenas um animal é afetado. Neste relato, duas éguas, de sete e nove anos, foram diagnosticadas concomitantemente com mastite crônica piogranulomatosa, associada com reação de Splendore - Hoeppli, indicativa de botriomicose, uma doença crônica supurativa rara, com formação de microabscessos caracterizados pela presença de material eosinofílico em torno dos microrganismos ou de material biologicamente inerte. Várias bactérias podem causar botriomicose em cavalos, sendo Staphylococcus sp., particularmente S. aureus, as mais frequentemente isoladas. Este relato confirma o papel do Staphylococcus sp, no entanto este é o primeiro relato em que S. hyicus e S. cohnii foram identificados em lesões relacionadas à botriomicose.(AU)

Factores de riesgo para endometritis puerperal en pacientes que asisten para terminación del embarazo en el hospital Simón Bolivar entre enero 2007 a diciembre 2013 / Risk factors for puerperal endometritis in patients attending for termination of pregnancy at Simón Bolivar hospital between january 2007 to december 2013

La endometritis Posparto constituye la causa más frecuente de fiebre puerperal, y su incidencia aumenta con la presencia de ciertos factores de riesgo que se pueden encontrar en todos los niveles de atención de la gestante y su identificación, permitiría disminuir la morbimortalidad en los servicios de Ginecología y Obstetricia. OBJETIVO Determinar los factores de riesgo para endometritis puerperal en el servicio de Ginecología y Obstetricia del Hospital Simón Bolívar. MATERIALES Y MÉTODOS Se realizó un estudio observacional tipo analítico retrospectivo de casos y controles en el Hospital Simón Bolívar de la ciudad de Bogotá, en mujeres que asistieron durante el periodo comprendido entre Enero de 2007 a Diciembre de 2013 para terminación del embarazo durante el tercer trimestre. RESULTADOS Se analizaron un total 408 pacientes, 136 pacientes con endometritis postparto (casos) y 272 pacientes sin endometritis (controles), con una relación caso control de 1 caso por cada 2 controles. Al realizar el análisis de las variables a estudio como: paridad, ruptura de membranas, vía del parto, índice de masa corporal, revisión uterina, infección de vías urinarias y presencia de flujo vaginal, asociado a endometritis no mostraron resultados estadísticamente significativos. La asociación entre la edad de la madre, edad gestacional al momento del parto y preeclampsia evidenciaron un ligero aumento del riesgo para endometritis puerperal. CONCLUSIÓN Los principales factores de riesgo para endometritis puerperal encontrados entre las pacientes atendidas por servicio de Ginecología y Obstetricia del Hospital Simón Bolívar fueron gestantes de edad avanzada, pacientes con preeclampsia asociada y edad gestacional menor de 37 semanas al momento del parto. La vía de parto y la atención intraparto no tuvieron asociación ni aumento del riesgo de forma significativa.

Equilibrio Ácido-Base: el mejor enfoque clínico / Acid-base equilibrium: The best clinical approach

Acid-base balance disorders can be found in a primary or secondary form in patients with a disease process such as Diabetes Mellitus or acute renal failure, among others. The objective of this article is to explain and guide the correlation ship between the clinical findings in the patient and the parameters of arterial blood gases in a simple and precise manner, in order to make the correct acid-base balance diagnosis and adequate therapeutic interventions. A non-systematic review of the scientific literature was conducted through a search in the PubMed, Science Direct, Scopus, and OvidSP databases. The conclusion was that base excess or deficit in arterial blood gases is a useful tool which along with the clinical history, pH, and partial pressure of CO2, provides an accurate estimate of the metabolic component of the acid-base balance.

Las alteraciones del Equilibrio Ácido-Base se pueden presentar en pacientes de forma primaria o secundaria a un proceso patológico como la Diabetes Mellitus o la falla renal entre otros. El objetivo es explicar y orientar la correlación clínica del paciente con los parámetros de los gases arteriales de manera sencilla y precisa, para realizar un diagnóstico de las alteraciones del Equilibrio Ácido-Base correcto, que permita efectuar intervenciones terapéuticas adecuadas y oportunas. Se realizó una revisión no sistemática de la literatura científica en la cual se consultaron las siguientes bases de datos: PubMed, ScienceDirect, Scopus y OvidSP en busca de artículos relevantes. Se concluyó que el exceso o déficit de base es una herramienta útil de los gases arteriales, que aunada a la historia clínica, el pH y la presión parcial de CO2 estima de forma muy precisa el componente metabólico del Equilibrio Ácido-Base.

Patrones de prescripción del ácido acetilsalicílico / Prescribing patterns of acetylsalicylic acid

Objetivo: Determinar los patrones de prescripción del ácido acetilsalicílico, además de los costos y variables asociados a su utilización en una población de pacientes afiliados al Sistema General de Seguridad Social en Salud (SGSSS) de Colombia en el año 2014. Métodos: Estudio descriptivo de corte transversal. Mediante una base de datos de aproximadamente 3,5 millones de pacientes afiliados al SGSSS de Colombia en 88 ciudades, se seleccionaron pacientes a quienes se les dispensó ácido acetilsalicílico entre el 1.° de enero el30 de marzo de 2014 de manera ininterrumpida. Se analizaron variables sociodemográficas, farmacológicas y de comedicación. Resultados: Un total de 69.807 pacientes recibieron aspirina durante el periodo evaluado, con una edad promedio de 66,7 ± 12,1 años; el 57,1% eran hombres; el 99,8% tomaban alguna comedicación: el 94,8% antihipertensivos; el 22,4% antihipertensivos, antidiabéticos e hipolipemiantes de manera concomitante; el 95,4% estaban en monoterapia con aspirina, y el 4,6% recibían terapia combinada con 2 o más antiagregantes plaquetarios. El análisis multivariado encontró mayor probabilidad de recibir terapia combinada en Bogotá, Barranquilla y Cartagena. La dosis usual fue 100 mg/día y la dosis diaria, definida por 1.000 habitantes/día, fue 19,9. Conclusiones: La prescripción de ácido acetilsalicílico en Colombia se realizó a las dosis recomendadas por la Organización Mundial de la Salud para la prevención del riesgo cardiovascular, y su proporción de uso es inferior cuando se compara con la de otras poblaciones.

Objective: Determine the prescribing patterns of acetylsalicylic acid, costs and variables associated with its use in a population of patients affiliated to the Colombian Health System in 2014. Methods: Cross-sectional descriptive study. Using a database of approximately 3.5 million patients beneficiaries of the Colombian Health System in 88 cities; patients who were dispensed with acetylsalicylic acid continuously since 1st of January to 30th of March 2014 were selected. Sociodemographic, co-medication and pharmacologic variables were analyzed. Results: A total of 69,807 patients were receiving aspirin during the assessment period with a mean age of 66.7 ± 12.1 years; 57.1% were male; 99.8% received some co-medication: 94.8% received antihypertensive agents; 22.4% were receiving antihypertensive, antidiabetic and lipid-lowering drugs concomitantly; 95.4% were receiving monotherapy with aspirin, and 4.6% received combination therapy with 2 or more antiplatelet agents. Multivariate analysis found that it was more likely to receive combination therapy in Bogota, Barranquilla and Cartagena. The usual dose was 100 mg and defined daily dose/1,000 inhabitants/day was 19.9. Conclusions: The prescription of acetylsalicylic acid in Colombia was made at recommended doses by the WHO for the prevention of cardiovascular risk; and their frequency of use was lower when compared with other populations.

Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats

The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats (P<0.01). During the ischemia period, there was an increase in the QRS interval (P<0.05) and a reduction in the P wave and QT intervals (P<0.05 for both) in rats with epilepsy compared to control rats. During the reperfusion period, a significant reduction in the QT interval (P<0.01) was verified in the epilepsy group in relation to the control group. Our results indicate that rats submitted to an epilepsy model induced by pilocarpine presented electrical conductivity alterations of cardiac tissue, mainly during an AMI episode.

Seguro Popular, condiciones psicosociales de trabajo y violencia en empleados de una institución de salud en méxico: un análisis desde el modelo de los determinantes sociales de la salud / Popular Health Insurance, psychosocial factors at workplace, and violence in employees from a health institution in Mexico: an analysis from the model of the social determinants of health

OBJETIVO : analizar el impacto de la implementación y operación del Seguro Popular en las condiciones psicosociales de trabajo y la exposición a situaciones y comportamientos de violencia de un grupo de trabajadores que laboran en una institución de salud. METODOLOGIA: se realizó un estudio cualitativo bajo el marco de la sociología fenomenológica. Se aplicó una encuesta a 206 trabajadores para valorar las condiciones psicosociales negativas en el entorno laboral. Posteriormente, se identificaron 18 sujetos caso por haber obtenido puntajes negativos altos en la encuesta y haber estado expuestos o haber participado en situaciones y comportamientos de violencia en el trabajo. RESULTADOS Y DISCUSIÓN :la implementación y operación del Seguro Popular provocó un deterioro y precarización de las condiciones psicosociales y del proceso de trabajo. La exposición a situaciones y comportamientos de violencia se manifestó como estrés , desgaste emocional, pérdida de la motivación, minusvalía y depresión. CONCLUSIONES : las reformas laborales y de la administración pública, expresadas en un nuevo modelo de gestión organizacional orientado a la eficiencia y reducción de costos prioriza los criterios administrativos y desestima la satisfacción y bienestar de los trabajadores.

OBJECTIVE: to analyze the impact of the implementation and operation of the Popular Health Insurance underwork psychosocial conditions and violence of a group of workers from a health institution. METHODOLOGY a qualitative study was realized under the framework of the phenomenological sociology. A survey was applied to two hundred six workers for the evaluation of the negative psychosocial conditions in the work environment. Later, it was identified to eighteen individual «cases¼for having obtained high negatives scores in the survey and having been exposed or for having taken part in situations and behaviors of violence at workplace. RESULTS AND DISCUSSION:the implementation and operation of the Popular Health Insurance provoked deterioration and precarious psychosocial conditions and work process alterations. Violence at workplace expressed like distress, emotional exhaustion, loss of motivation, disability and depression. CONCLUSIONS : labor and public administration, reforms in a new model of organizational management oriented to efficiency and cost reductions, prioritizes the administrative criteria and rejects the satisfaction and subjective well-being of workers.

Treatment of Wastewaters Generated by Surfactant-Enhanced Washing of Soils in an Aerobic Biofilter Inoculated with a Consortium of Hydrocarbon Degraders.

Aims: The aims of this work were 1) To evaluate the performance of a submerged biofiltration system for the treatment of a surfactant-enriched wastewater that had been generated by a soil washing process. 2) To evaluate the effect of the flux and organic load over the performance of the system. 3) To determine the microbial evolution as an effect of the flux at different lengths of the biofilter by using a denaturing gradient gel electrophoresis (DGGE) analysis. Study Design: A three factorial design was used to evaluate the effect of different fluxes and organic loads over the performance of a continuously operated submerged aerobic biofilter. The DGGE technique was employed to determine microbial changes in the biofilter. Place and Duration of Study: The study was carried out at the Bioprocess Laboratory, Bioprocesses Department UPIBI-IPN, Mexico. The experimental stage lasted approximately eight months and the DGGE analysis four months more. Methodology: Contaminated soil was physicochemical and microbiologically characterized. A total of 70 kg of contaminated soil was washed using a 1:3 ratio soil/surfactant solution (0.5% Sulfopon 30-SP30). The surfactant-enriched wastewater was then treated in a submerged biofilter. The biofiltration system consisted of a column with a length of 50 cm and diameter of 12 cm. The biofilter was packed with tezontle with an average diameter of 0.2-0.4 cm and 70% void space. The biofilter working volume was 4.5 L. The samples of the packing material for the DGGE analysis were obtained from the ports located along the biofilter: at the wastewater inlet, at the middle of the column and at the outlet. After DNA extraction with a Power Soil DNA Isolation Kit (MO BIO), PCR (polymerase chain reaction) analysis was conducted. The 16S rRNA gene was amplified using universal bacterial primers. The data obtained by DGGE analysis for the microbial population developed in the biofilter were further analyzed by the Jaccard similarity coefficient. Results: The soil contained 14,704 mg/kg TPH. BTEX compounds were not found, and only two different PAHs were found in the soil samples: benzo-fluoranthene and benzopyrene, at concentrations of 0.1280 and 0.0682 mg/kg of soil, respectively. During the surfactant-aided soil washing, the highest removal percentage of the oil removed from the soil was 59% with 0.5% SP30. The wastewater generated after the soil washing process contained, in average 1,329 mg COD/L and 211 mg/L of grease and oil. Higher COD removals were obtained at a flux of 0.4 L/h for both of the COD initial concentrations. While the highest removal was 78.27%, determined at an initial COD concentration of 300 mg/L. When applying fluxes of 0.28 and 0.40 L/h at a higher initial COD concentration, the COD removals were increased; this was not the case for a flux of 0.63 L/h. For a given initial COD concentration, the removal efficiencies were higher for lower fluxes. Analysis of the similarity between the microbial populations for varying fluxes and levels along the length of the biofilter was determined by the Jaccard (JI) index. The results showed that the initial microbial populations (t0) have low similarities with the developed microbial populations at the different conditions tested. Conclusion: Both the flux and the initial COD concentration had an impact on COD removal and the microbial concentration in the column. The COD removal percentages were similar at fluxes of 0.28 and 0.63 L/h. The highest removal percentage of 78.27% was obtained at a flux of 0.4L/h; this finding was in agreement with the highest microbial count and the specialization of microbial populations (less diversity). In general, it was shown that the flux had an effect on changes in microbial population. Greater effects were observed on the microbial population due to the position along the reactor, e.g., the greatest differences were found at the different levels of the biofilter.

Láser en ortodoncia / Laser in orthodontics

La primera aplicación del laser en un diente fue realizada en 1965. Desde entonces ha presentado una constante evolución y desarrollo. La tecnología laser permite realizar procedimientos en tejidos duros y blandos, pudiendo ser utilizado con las siguientes finalidades: como prevención de la desmineralización, en la adhesión y remoción de brackets, en la reducción del dolor producto del movimiento dental, en la reparación ósea después de la expansión, en diversas cirugías y otras aplicaciones más. El objetivo de este artículo es realizar una revisión bibliográfica sobre algunos de los usos, ventajas y características del laser en Ortodoncia.

The first application of laser in a tooth was made in 1965. Since then this tecnology had a constant evolution and development. Laser technology allows procedures in hard and soft tissues, it can be used for the following purposes: prevention of demineralization, bonding and debonding of brackets, to reduce pain resulting from tooth movement, bone repair after expansion, surgeries and other applications. The aim of this article is to review the literature on some of the uses, advantages and features of the laser in orthodontics.

Choroid plexus papilloma in a Rottweiler: computed tomographic, gross morfological and histological features / Papiloma do plexo coroide em um cão da raça Rottweiler: aspectos tomográficos, macroscópicos e histológicos

Among the tumors affecting the choroid plexus in dogs, the papilloma ranks second place in incidence after cell carcinoma tumors. Presumptive diagnosis can be made through imaging methods, such as computed tomography and magnetic resonance images. Definitive diagnosis of plexus choroid tumor is based on histopathological findings. This report presented the tomographic features of the brain in a 6-year-old intact female Rottweiler with choroid plexus papilloma. The computed tomography showed right lateral ventricle enlargement, midline deviation and an enhanced mass into the postcontrast phase. At necropsy, a mass on the floor of the right lateral ventricle was observed, associated with important ventricle dilatation. The histopathological analysis demonstrated the presence of neoplastic cell forms with papillary projections. The computed tomography proved to be an effective tool in the presumptive diagnosis of this kind of cerebral disorder.

Dentre os tumores que afetam o plexo coroide em cães, o papiloma figura como o segundo tipo de maior incidência, antecedido apenas pelo carcinoma. O diagnóstico presuntivo pode ser elaborado por meio de métodos de imagem, como a tomografia computadorizada e a ressonância magnética. O diagnóstico definitivo de tumor do plexo coroide é estabelecido com base nos achados histopatológicos. Relatamos os aspectos tomográficos do crânio em uma fêmea de 6 anos, inteira, da raça Rottweiler, com papiloma do plexo coroide. A tomografia computadorizada revelou dilatação do ventrículo lateral direito, desvio da linha média e a presença de uma massa, que sofreu realce na fase pós-contraste. À necropsia, foi observada uma massa sobre o assoalho do ventrículo lateral direito, associada à importante dilatação ventricular. A análise histopatológica demonstrou a presença de células poligonais neoplásicas, arranjadas em papilas longas. A tomografia computadorizada apresentou-se como uma ferramenta eficaz no diagnóstico presuntivo desse tipo de alteração cerebral.

Detección molecular de infecciones múltiples en mujeres con y sin lesión de cuello uterino positivas para el virus del papiloma humano de alto riesgo, período 2007-2011 / Molecular detection of multiple infections in women with and without cervical lesion positive for high risk human papillomavirus, period 2007-2011

El cáncer de cuello uterino, causado por el virus del papiloma humano (HPV), constituye el tercer tipo de cáncer más común en mujeres en el mundo. El objetivo del presente trabajo fue determinar por reacción en cadena de la polimerasa seguida de hibridación reversa, la frecuencia de infecciones múltiples en mujeres positivas para HPV de alto riesgo oncogénico por captura híbrida II, según severidad de la lesión escamosa intraepitelial (SIL), carga viral relativa y edad, en el periodo 2007-2011. Este estudio de corte transverso incluyó 123 mujeres que acudieron al Instituto de Investigaciones en Ciencias de la Salud, 18 sin lesión (NSIL), 78 con SIL de bajo grado (LSIL) y 27 con SIL de alto grado (HSIL). Se detectaron infecciones múltiples en 37/123 mujeres (30,1%). Las mujeres con NSIL presentaron menor frecuencia de infecciones múltiples (5,6%) que las mujeres con SIL (34,3%) (p=0,03). No se observó aumento de frecuencia de infecciones múltiples con la severidad de la lesión, presentando las mujeres con LSIL un 34,6% y con HSIL, un 33,3%. No fue observada diferencia significativa entre la carga viral relativa de infecciones simples y múltiples, lo cual sugiere que en la infección múltiple podría ser solo uno de los tipos de HPV el que se esté replicando, causando una infección productiva. Además, se observó una alta frecuencia de infecciones múltiples (75%) en mujeres menores a 30 años. Finalmente los resultados sugieren que las infecciones múltiples podrían orientar la presencia de SIL, pero no el grado de severidad de la lesión cervical.

Lactic acid production via cassava-flour- hydrolysate fermentation / Producción de ácido láctico vía fermentativa a partir de hidrolizado de harina de yuca

background: Lactic acid (LA) is a carboxylic acid widely used as preservative, acidulant, and/or flavouring in food industry; it is also used as a raw material for the production of lactate ester, propylene glycol, 2,3-pentanedione, propanoic acid, acrylic acid and acetaldehyde. In recent years, the demand for LA production has dramatically increased due to its application as a monomer for poly-lactic acid synthesis, a biodegradable polymer used as a plastic in many industrial applications. LA can be produced either by fermentation or chemical synthesis; the former route has received considerable interest, due to environmental concerns and the limited nature of petrochemical feedstocks; thus, 90% of LA produced worldwide is obtained by fermentation, this process comprises the bioconversion of a sugar solution (carbohydrates) into LA in the presence of a microorganism. Objectives: This work is aimed at studying the effect of pH control and culture media composition on the LA production using renewable sources from the agroindustry sector. Methods: A Lactobacillus brevis strain is used to perform lab scale experiments under aerobic and anaerobic conditions, using three different culture media compositions: a high nutritional content medium (MRS), as a reference, a low nutritional content medium with glucose as the only carbon source (GM), and a potential low nutritional content medium with cassava flour as carbon source (HY1). results: The higher LA production is accomplished under anaerobic conditions, 17.6 ± 0.1, 12.6 ± 0.2 y 13.6 ± 0.2 g LA/L, for MRS, GM and HY1 medium, respectively. The effect of pH on LA biosynthesis in a 5L bioreactor is also studied using the HY1 medium. For a fermentation time of 120 h, the highest LA concentration obtained was 24.3 ± 0.7g LA/L, productivity 0.20 g/L/h, YP/S 0.32g LA/g syrup, at pH 6.5...

Association between oral health-related quality of life and atraumatic restorative treatment in school children: An exploratory study.

Background: The present study's objectives were to compare the perceptions of oral health-related quality of life (OHRQoL) among school children presents dental caries with that of caries-free school children, and to evaluate the subjective impact of atraumatic restorative treatment (ART) on the OHRQoL of school children. Design: Exploratory study. Setting: Public school in Piracicaba, Brazil. Materials and Methods: The sample was composed of 30 children in the age-group of 8-10 years. The children were divided into two groups: one with caries and other without caries. The information related to OHRQoL was obtained through the administration of the Child Perceptions Questionnaires (CPQ 8-10 ). To evaluate the impact of ART on the school children's OHRQoL, the CPQ 8-10 was re-administered 4 weeks after the initial treatment. For statistical analysis we used the nonparametric Wilcoxon test for two dependent variables (test and re-test groups) and the nonparametric Mann-Whitney test for independent variables (test and control groups). Results: It was observed that children with dental caries reported larger impact of oral health conditions on the quality of life when compared with the caries-free group, especially in the emotional and social well-being domains ( P≤.05). After the school children who presented with dental caries received ART, there was improvement in the median scores of CPQ 8-10 in all of the domains, but particularly in the functional limitation domain ( P≤.05). Conclusion: Dental caries exerts a strong influence on the OHRQoL of children. ART was shown to be a simple and painless caries management approach that could improve the OHRQoL of school children.

Estudio de la mdduración de carne de cordero empleando electroforesis sds-page / Study of lamb meat ageing using electrophoresis sds-pgee / Estudo domaturaçção cordeiros usando eletroforese sds-page

La maduración de la carne es causada por reacciones metabólicas que producen cambios en su calidad, mejorando las características organolépticas como la terneza, la jugosidad y el color. El presente estudio tuvo como objetivo encontrar los cambios producidos en las proteínas sarcoplasmáticas y miofibrilares durante la instauración del rigor mortis y la maduración en carne de cordero semiestabulado (AS) y cordero alimentado por pastoreo (AP). Para tal fin se tomaron muestras del músculo Longissimus dorsi de corderos de raza Dorper x criollo ¾ de diez semanas de edad. Las proteínas sarcoplasmáticas se extrajeron con agua y solución salina diluida, y las proteínas miofibrilares con solución salina concentrada. La cuantificación se hizo por el método de Bradford y la separación por electroforesis SDS-PAGE. En las proteínas sarcoplasmáticas se observó la aparición de una banda de 146 kDa a los cinco y trece días para los corderos AS y AP, respectivamente. En las proteínas miofibrilares se encontró evidencia del rigor mortis a las 16 h y una intensificación drástica de una banda de 28 kDa a las 16 h en el cordero AS. Esta banda también se observó en el cordero AP a las 14 h. En todo el proceso se pudo ver la degradación gradual de la DESMINA, ACTINA Y TROPONINA T.

The ageing of meat is caused by metabolic reactions than result in changes in its quality, improving its organoleptics characteristics such as the tenderness, the juiciness and the color. This study had the aim to find changes in sarcoplasmic and myofibrillar proteins during the establishment of rigor mortis and the ageing in lamb meat: semi feedloot (SF) and lamb feeding for pasture (FP). For this purpose, were sampled Longissimus dorsi muscle of lambs bred Dorper x Criollo ¾ and 10 weeks old. Sarcoplasmic proteins were extracted with water or saline solution diluted and the myofibrillar protein with saline solution concentrate. The Quantification was done by the Bradford method and the separation by SDS-PAGE electrophoresis. In the sarcoplasmic proteins observed the appearance of a band of 146 kDa in the samples of five and thirteen days for lamb SB and AP, respectively. In the myofibrillar proteins found evidence of the establishment of rigor mortis at 16 hours and a drastic intensification of a band of 27 kDa to 16 hours in the lamb SB. This band was also observed in the lamb FP at 14 hours. In the whole process could see the gradual degradation of Desmin, Actin and Troponin T.

A maturação da carne é causada por reações metabólicas que resultam em alterações na qualidade, melhorando as características organolépticas, tais como a suculência, ternura e cor. Este estudo teve como objetivo encontrar alterações nas proteínas miofibrilares sarcoplasmático e durante o início do rigor mortis e maturação na carne: cordeiro Semiestabulado (AS) e pastagem-fed cordeiro (AP). Para o efeito, amostras do músculo Longissimus dorsi de cordeiros Dorper x criollo ¾ de 10 semanas de idade. As proteínas sarcoplasmático foram extraídas com água ou soro fisiológico e diluído com concentrado de proteína miofibrilar salina. Quantificação foi feita pelo método de Bradford e separação por electroforese SDS-PAGE. Nas proteínas sarcoplasmático observado o aparecimento de uma banda de 146 kDa cinco e 13 dias de cordeiro AS e AP, respectivamente. As proteínas miofibrilares encontraram evidências de rigor mortis em 16 horas e uma intensificação drástica de uma banda de 28 kDa a 16 horas no cordeiro AS. Esta banda também foi observada na AP cordeiro em 14 horas. Durante todo o processo podia ver a degradação gradual da Desmina, Actina e Troponina T.

Polyethylene Wrap for Thermoregulation in the Preterm Infant: A Randomized Trial.

We compared the response of temperature adaptation in preterm infants using the polyethylene wrap with and without previous drying versus the sterile preheated field. Both groups of polyethylene use achieved a mean axillary temperature of 36.5°C at 30 minutes compared with 75 minutes for the group of traditional care. At 120 minutes, the incubator temperature was higher in those using preheated field, compared with infants in the polyethylene wrap with or without previous drying, (35.15°C, 34.20°C and 34.20°C respectively; P = 0.0001). No difference in axillary or incubator temperature was found between the groups using the polyethylene wrap.

Perfiles de personalidad de estudiantes de psicología de universidad privada comparados em similitud y congruencia / Personality profiles of psychology students from a privateuniversity compared by similarity and congruence

En continuidad con los estudios del área de Perfiles de la Cátedra de Psicología Experimental I y II, se llevó a cabo un análisis comparativo de Perfiles de Personalidad en estudiantes de psicología. La muestra fue intencional y autoseleccionada, quedó conformada por 153 estudiantes de Psicología. Se ha utilizado un diseño Descriptivo y Comparado. El instrumento utilizado fue el Inventario Multifásico de la Personalidad Minnesota-2 (MMPI-2). Los resultados reportan que existe un patrón de personalidad con similitudes muy acentuadas en los estudiantes de la carrera de psicología, independiente del curso al que pertenecen, y en comparación a los perfiles de años anteriores (n = 152, muestra 2001: n=168, muestra 2007: y n= 101 muestra 2008).

In continuity with the studies of the area of Profiles of the Professorship of Experimental Psychology I and II, a comparative analysis of Profiles of Personality in students of psychology was carried out. The sample was intentional and autoseleccionada, remained conformed by 153 (166) students of Psychology. A Descriptive design has been utilized and Compared. The instrument utilized was the Polyphase Inventory of the Personality Minnesota-2 (MMPI-2). The results report that a boss of personality with similarities exists very accentuated in the students of the career of psychology, independent of the course to which they belong, and in comparison to the profiles of previous years (n = 152 sample 2001; n = 168, sample 2007 and n = 101 sample 2008).

Evaluation of the immune humoral response of Brazilian patients with Rubinstein-Taybi syndrome

Rubinstein-Taybi syndrome (RTS) is a rare developmental disorder characterized by craniofacial dysmorphisms, broad thumbs and toes, mental and growth deficiency, and recurrent respiratory infections. RTS has been associated with CREBBP gene mutations, but EP300 gene mutations have recently been reported in 6 individuals. In the present study, the humoral immune response in 16 RTS patients with recurrent respiratory infections of possible bacterial etiology was evaluated. No significant differences between patients and 16 healthy controls were detected to explain the high susceptibility to respiratory infections: normal or elevated serum immunoglobulin levels, normal salivary IgA levels, and a good antibody response to both polysaccharide and protein antigens were observed. However, most patients presented high serum IgM levels, a high number of total B cell and B subsets, and also high percentiles of apoptosis, suggesting that they could present B dysregulation. The CREBBP/p300 family gene is extremely important for B-cell regulation, and RTS may represent an interesting human model for studying the molecular mechanisms involved in B-cell development.

Aplicación de un programa de entrenamiento mnemotécnico para incrementar la memoria en adultos mayores / Application of a program mnemonic training to increace the memory in major adults

La investigación busca comprobar que por medio de la aplicación del programa de entrenamiento Mnemotécnico (VI) es probable incrementar la memoria (VD) de un grupo de adultos mayores de sexo masculino, cuya edad oscila entre 70 y 75 años. La muestra intencional y autoselectiva será de no más de veinte participantes. Se aplicará un diseño cuasi-experimental intragrupo pre-post-evaluación, durante el cual se evaluará pre-post la memoria inmediata de los adultos mayores mediante una batería de test. El paradigma a utilizar será el O1 X O2. Para realizar el análisis de datos se aplicará estadística descriptiva e inferencial empleando la t de Student, con un nivel de significación de α: 0.05.

Avaliação ultra-sonográfica e pelo Doppler colorido do carcinoma de células transicionais da bexiga em cães / Ultrasonography and color Doppler investigation of transicional cell carcinoma of the bladder in dogs

Estudaram-se os aspectos ultra-sonográficos das lesões sugestivas de carcinoma de células transicionais (CCT), investigou-se a contribuição do mapeamento Doppler colorido e de amplitude no diagnóstico das lesões sugestivas de CCT e verificou-se a acurácia do método de biópsia ecodirigida por sucção traumática via sondagem uretral na coleta de material para confirmação diagnóstica CCT da bexiga. Para tal, foram selecionados nove cães que atendiam aos critérios de inclusão determinados. Destes, sete apresentavam CCT. A ultra-sonografia permitiu a detecção de massas exofílicas com características vegetantes em cinco cães, e de lesões infiltrativas na parede vesical estendendo-se para a uretra em dois. Pelo exame com Doppler colorido e de amplitude, constatou-se a vascularização interna da massa, indicativa de neoangiogênese tumoral, em quatro animais. A técnica de sucção traumática por aspiração permitiu a elucidação diagnóstica em cinco dos sete animais com CCT. Concluiu-se que, na maioria dos casos, a ultra-sonografia propicia a identificação de lesões sugestivas de CCT, constituindo a primeira técnica de diagnóstico por imagem indicada para pacientes que apresentem hematúria e disúria

The aims of this study were to evaluate the ultrasonographic findings in dogs with suggestive transitional cell carcinoma (TCC) lesion, to investigate the use of the color Doppler for the diagnosis of that condition and to establish the efficacy of the ultrasound catheter biopsy by suction via urethral probing for collecting material to confirm bladder TCC. A total of nine dogs were selected, seven with TCC confirmed. The ultrasonographic finding in TCC patients detected sessile masses in the bladder of five dogs and infiltrative mural lesion extent to the urethra in two dogs. The color Doppler and the power Doppler showed the internal vascular flow in four animals indicating tumor neoangiogenesis. It was concluded that the ultrasonography may identify abnormalities in the majority of cases with suggestive lesions of TCC and the ultrasonographic evaluation should be considered as the first diagnostic imaging modality in patients presented with hematuria and dysuria