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Rev. med. (Säo Paulo) ; 84(2): 82-89, 2005. ilus
Article in Portuguese | LILACS | ID: lil-419611

ABSTRACT

Este trabalho apresenta como objetivo testar o cultivo de condrócitos por um método não enzimático. Para isto, foram isolados condrócitos de cartilagem costal humana pelo método de explante primário, em que as células migraram da cartilagem e se aderiram ao fundo do frasco de cultura. posteriormente, pelo método de imunofluorescência indireta dosou-se a capacidade de produção de colágeno tipo II pelos condrócitos isolados a fim de / This work presents as objective to test culture of chondrocytes for a not enzymatic method of primary explante, where the cells migrate of the cartilage and if they had adhered to the deep one in the bottle of culture. Later, for the method of indirect immunofluorescence it was dosed capacity of production of collagen type II for the isolated chondrocytes in order to evaluate the capacity of the cells to produce extracellular matrix to demonstrate the maintenance of the physiological capacities of the isolated cells in culture...


Subject(s)
Cartilage, Articular/injuries , Collagen Type II/therapeutic use , Chondrocytes , In Vitro Techniques , Cartilage/injuries , Cartilage Diseases/therapy
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