ABSTRACT
This paper has reported the preliminary phytochemical screening, HPTLC analysis of phytocompounds and in vitro antioxidant activities of ethanol extract of Eugenia singampattiana leaves. This is the first report on the antioxidant activity of this plant. The preliminary phytochemical analysis showed the presence of alkaloids, coumarin, catechin, steroids, flavonoids, saponins, phenols, glycosides and terpenoids. HPTLC analysis also confirmed the presence of alkaloids, steroids, flavonoids, saponins, phenols, glycosides and terpenoids. The antioxidant activities of the leaves in ethanol extract are assessed using different models like DPPH, superoxide radical, hydroxyl radical and ABTS+ cation radical and reducing power at different concentrations. The ethanol extract at 800μg/ml showed maximum scavenging activity. Results obtained revealed that, ethanol extract of leaves of E. singampattiana possess high antioxidant activity. Thus this study suggests that, E. singampattiana plant can be used as a potent source of natural antioxidant.
ABSTRACT
The present investigation was carried out to determine the possible bioactive components of whole plant of Hedyotis leschenaultiana using GC-MS analysis. Fourteen components from the whole plant of the above said plant were identified. The prevailing compounds in the ethanol extract were 9-Octadecenoic acid (z)-, Methyl ester (22.57%), β-Sitosterol (17.65%), 3,7,11,15-Tetramethyl-2-hexadecenl-o1 (12.29%), Phytol (8.61%), Oxirane, (butoxymethyl)- (8.04%), Squalene (6.48%), 1,6 Anhydro- á – D- glucopyranose (levoglycosan) (5.03%), 9-12-Octadecadienoic acid (z,z)- (4.47%), Eicosane (3.35%), 1-Eicosanol (3.35%) and Diazoprogesterone (2.91%). This is the first report of identification of active constituents from the whole plant of Hedyotis leschenaultiana. This work will help to identify the compounds of therapeutic value.