ABSTRACT
Objective To investigate the inhibition effects of "Phrul sByor Chem Mo (PBCM)" against different common digestive system tumor cell lines in vitro, including human gastric carcinoma cell line MGC803, colorectal carcinoma cell line LoVo, and hepatocellular carcinoma cell line HepG2. Methods The digestive system tumor cell lines in the logarithmic growth phase were incubated with different concentrations of PBCM for 48h. The cell viability was investigated by MTT assays, apoptosis was detected by Hoechst 33342 assays, and the level of Bcl-2 and Bax was estimated by ELISA tests. Results MTT assays proved that the proliferation of digestive system tumor cell lines was significantly inhibited by PBCM in vitro. Fluorescent staining demonstrated that PBCM was able to promote apoptosis of the cell lines. ELISA tests illustrated that the level of proapoptotic Bax was elevated, while the value of antiapoptotic Bcl-2 was decreased. Conclusion PBCM is able to inhibit proliferation and induce apoptosis of digestive system tumor cell lines in vitro. The mechanism may be related with Bcl-2 family proteins.
ABSTRACT
Objective To investigate the inhibition effects of "Phrul sByor Chem Mo (PBCM)" against different common digestive system tumor cell lines in vitro, including human gastric carcinoma cell line MGC803, colorectal carcinoma cell line LoVo, and hepatocellular carcinoma cell line HepG2. Methods The digestive system tumor cell lines in the logarithmic growth phase were incubated with different concentrations of PBCM for 48h. The cell viability was investigated by MTT assays, apoptosis was detected by Hoechst 33342 assays, and the level of Bcl-2 and Bax was estimated by ELISA tests. Results MTT assays proved that the proliferation of digestive system tumor cell lines was significantly inhibited by PBCM in vitro. Fluorescent staining demonstrated that PBCM was able to promote apoptosis of the cell lines. ELISA tests illustrated that the level of proapoptotic Bax was elevated, while the value of antiapoptotic Bcl-2 was decreased. Conclusion PBCM is able to inhibit proliferation and induce apoptosis of digestive system tumor cell lines in vitro. The mechanism may be related with Bcl-2 family proteins.