ABSTRACT
In the study, about 695 samples comprising (fish powder: 320 samples, blood meal: 41 samples, bone meal: 123 samples), finished feed (pellets of pig feed: 213 samples) were collected and detected by polymerase chain reaction (PCR). Isolation prevalence in fish powder, blood meal, bone meal, finished feed was 23(7.19 %), 9 (21.95%), 48 (39.67%), 2 (0.94%) respectively. These Salmonella showed different antibiotic sensitivities to erythromycin, ampicillin, penicillin, ciprofloxacin. However, all these strains were inhibited with plantaricin produced by Lactobacillus plantarum PN05 isolated in Coryandrium sativum. Our findings highlighted a potential public health hazard and warned human the outbreaks of human salmonellosis with high resistance due to the consumption of contaminated feed and also suggested the prevention by plantaricin of Lactobacillus plantarum PN05.
ABSTRACT
Bacillus subtilis spores were used to study on drug delivery nowadays. However, evaluation on the cisplatin packaging and releasing ability in spores is still limited. In this study, we determined Bacillus subtilis spores (2x108) could be packaged to 54,600,000 drug molecules of cisplatin from 8 to 12 hours. Spores were checked by scanning electron microscope and transmission electron microscope, and then drug release was done. Cisplatin started releasing at 0.5 hours at pH 3.4 (23.8%) that was higher than pH 7.2 (0.5%). Cisplatin released irregularly during 24 hours in pH 3.4. In contrast, the cisplatin release was slow and steady during 24 hours in pH 7.2. The release ability was only 25% that meant that cisplatin packing spores would be used in prolonged-release finished products. The study was the first report of the cisplatin-packaged spore and release ability of cisplatin out of spore that could be used in pharmaceutical field.