A method for determination of trace content of protein in material of chitin/chitosan

Protein trace present in chitin/chitosan product has been determined by our proposed method in which protein trace included in chitin/chitosan was extracted by refraction in 10% NaOH solution at 100oC for 5 hours. Chitin/Chitosan precipitate was discarded. protein trace in the solution was restored by neutralizing with HCl 10N to get the yellow powder. This powder was then analyzed for total nitrogen content by Kjeldahl method. The control method was conducted at the same procedure excepted from the presence of 10% NaOH. The results of different chitin/chitosan samples obtained from these two methods showed that amount of protein trace obtained from above proposed method was somewhat higher than that of the control one. Besides, the purified chitosan sample gave the lower protein trace content than that of the technical chitosan sample as well as chitin one. Protein trace bound between macromolecules of chitin/chitosan was separated in the proposed method by boiling water under alkaline condition whereas it was not completely separated in the control method because sodium hydroxide was absent.

Study on synthesis of polymeric drug: Synthesis of antimalarial drug with delayed effect and natural origin

The coupling procedure of antimalarial agent (SR2) onto polymeric carrier (SR1) by covalent bond (amide linkage) was studied. Some factors of drug coupling reaction was investigated.

Preliminary results of study on manufacture of biological polymeric membrane for the treatment of burns

Biopolymeric membrane is made on the polysaccharide of natural origin according to our own technological process. It is a high bioactive, biocompatible, biodegradable polymeric membrane with effective action for treating wound healing. The preliminary results of clinical study suggested that the membrane were effectively used as biologic dressings for the treatment of donor sites of skin graft, partial thickness burns and granulation tissue. The wound surface after healing was smooth and flexible. The biopolymeric membrane is not only suitable for the treatment of burn wounds, but also it can applied for the skin surgery with excellent results.