ABSTRACT
Objectives: Prolidase plays a vital role in collagen turnover, matrix remodeling, and cell growth. We aimed to evaluate the association between treatment with chorionic gonadotropin and infertility and erectile dysfunction by investigating
tissue prolidase activity, oxidative stress, and levels of antioxidant enzymes
Materials and Methods: The 16 male Wistar albino rats used in this study were randomly divided into 2 groups: rats treated with human chorionic gonadotropin [hCG] and control rats [n = 8 in each group]. The rats in the hCG group were subcutaneously injected with 50 IU hCG daily for 15 days, while the rats in the control group were subcutaneously injected isotonic saline. All of the rats were sacrificed by a lethal overdose of sodium pentobarbital at the first month after hCG administration. Prolidase activity and levels of malonyl aldehyde, glutathione reductase, superoxide dismutase [SOD], glutathione peroxidase [GSH-Px], and catalase [CAT] were estimated in the testicular and penile tissue. The testicles and penis were transversely dissected and placed in formalin
Results: Levels of prolidase and malonyl aldehyde in the testicular and penile tissues were significantly higher in the hCG group than in the control group [p < 0.001], while levels of glutathione reductase, SOD, GSH-Px, and CAT were significantly lower in the hCG group than in the control group [p < 0.001]
Conclusions: In this study, we observed that treatment with hCG increased prolidase activity and oxidative stress and decreased the antioxidant capacity of penile and testicular tissues; therefore, this may affect fertility and erectile function
ABSTRACT
Background: infertility is described as not receiving pregnancy despite unprotected and regular sexual intercourse in a 1 year period. It is detected by 15% of the couples. Male and female factor in the etiology may be detected in similar rates
Objective: the present study aims to investigate ion channel gene expression in semen samples of infertile male compared with fertile men
Materials and Methods: a total of 150 men who applied to the urology clinic due to infertility were divided into five equal groups: asthenozoospermia, oligozoospermia, oligoasthenoteratozoospermia, teratozoospermia, and normozoospermia [control]. All participants were evaluated with Cation Channel Spermia [CatSper] 1, 2, 3, 4, Proton Voltage Gated Ion Channel1 [Hv1], Potassium Channel Subfamily U1 [KCNU1], and transmembrane protein [TMEM16A] gene expression in semen samples
Results: "CatSper1, 4, HV1, KCNU1, and TMEM16A gene expression were detected higher in the oligozoospermia group compared to the controls. CatSper1, 2, 3, 4, KCNU1, and TMEM16A gene expression in the asthenozoospermia group and CatSper1, 2, 3, 4, KCNU1, and TMEM16A gene expression in the teratozoospermia group were detected lower compared to the controls. CatSper1, 4, HV1, and TMEM16A gene expression were higher in the oligoasthenoteratozoospermia men than the controls while CatSper3 gene expression was detected as lower."
Conclusion: it was detected that these ion channels have an effect on sperm progressive motility and morphology. It may be considered that mutations in these ion channels may result in infertility