ABSTRACT
Background@#Soil pollution in Ulaanbaatar has increased in recent years, causing infectious and non-communicable diseases, both benign and malignant. </br> Therefore, in order to reduce soil pollution, it is necessary to isolate strains resistant to heavy metals and use these microorganisms capable to degrade or accumulate heavy metals for bioremediation purposes.@*Methods@#Bacterial pure cultures were isolated from soil and water samples and incubated in a Nutrient Broth medium with a lead concentration of 1000 mg/L in a thermostat with a shaker at 37°C. The growth rate was determined at a wavelength of 600 nm using a Spectrophotometer.@*Results@#In total 69 pure cultures were isolated from the soil samples and 65 pure cultures from them were able to grow at the concentration of lead of 1 mmol/L. 4 pure cultures (UBLF1, UBLF3, UBMF2, and SUBZ4) were able tolerate lead concentrations up to 8 mM. These cultures were determined by semi-automatic VITEK®2 analyzers and identified as Bacillus sp. (83%) and UBMF2 identified as Bacillus thuringiensis (93%) respectively.@*Conclusion@#The lead content in water samples were found to be acceptable, and isolated pure cultures were not able to withstand the lead concentrations.</br> Pure culture UBLF3 isolated from the soil capable to reduce lead concentration (500 mg/L) up to 302 mg/L and 230 mg/L in the medium, and pure culture UBMF2 up to 340 mg/L and 279 mg/L respectively
ABSTRACT
1.Changes in microorganism’s quantity or activity can precede detectable changes in soil chemical properties, thereby providing an early sign of soil improvement or early warning soil degradation.2.Erdenet mining factory soil has approsintly 0.59*106 cell/g and control soil has 1.17*106 cell/g. Herein bacteria were 57%, actinomycete 31% and fungi 12%. But control soil bacteria were 83%, actinomycete 16% and fungi 1%.3.We have chosen deferent by morphology separate 29 cleanly cultures. In tolerance of heave metal, cultures which extracted from 1415 m level is tolerant against 6 mM of Fe, 5 mM of Pb, 3 mM of Mo and Cu, 1 mM of Cr