ABSTRACT
Background:Practice of hand washing is very important to eliminate the microbial contamination especially during the work in laboratories, hospital and even at home before taking food. Proper use of hand sanitizer can significantly reduce the rate of hospital acquired infection also. Methods:The current investigation was designated to identify different bacterial species from the upper skin of hands of the laboratory managements through conventional culture methodsand the efficacy of the samples (Dettol, Purell and Savlon) against the isolated bacteria throughagar well diffusion method and minimum inhibitory concentration (MIC)Results:Different concentrationssuch as 25%, 50% and100% of eachof antimicrobial agentsshowed their antibacterial activity againstStaphylococcus spp., Klebsiellaspp.,E. coliand Pseudomonasspp. those were isolated from the hand.25% of Dettolexhibited 20mm zone diameter against Klebsiellaspp. Whereas 25% of Purell and Savlon unveiled 15mm and 22mm zone of inhibition against E. coliand Pseudomonasspp. respectively. In case of 100% sample of Dettol, Purelland Savlon, the highest zone diameter was observedas 55mm, 50mm and45mm against Klebsiellaspp.,Staphylococcus spp., and E. coliconsecutively. The MIC of Dettol sample was 8μl against Staphylococcus spp., and Pseudomonasspp.In case of Purell, the MIC was 128μl againstStaphylococcusspp., Klebsiellaspp. and Pseudomonas spp. were inhibited at 4μl samples.Conclusions:The in-vitro antibacterial activity of the hand sanitizers was so satisfactory against the isolated bacteria. This finding would be very helpful for the laboratory management in order to minimize the rate of contamination during the research and supervision of the different experiment.