ABSTRACT
To investigate the effects of enteral and parenteral glutamine [Gln] usage on rats in sepsis. This study was conducted in Istanbul University Experimental Medical Research Institution [DETAE] laboratory, Istanbul University, Istanbul, Turkey between June and September 2009. The levels of blood Gln, tumor necrosis-alpha [TNF-alpha and interleukin [IL]-10 was measured. Samples of tissue were obtained from the mesenteric lymph nodes, liver, and lower lobe of the right lung to evaluate the reproduction of bacteria, and samples of tissue were also obtained from the small intestine to evaluate blood and villus atrophy. Bacteremia of Group EP [combined group] were found lower than Group C [control] [p=0.007]. Villous atrophy rates of all groups were lower than Group C: between Group E [enteral] and C [p=0.003]; between Group P [parenteral alanine] and C [p=0.019]; and between Group EP and C [p=0.001]. The values of serum TNF-alpha and IL-10 of Group EP and P were lower than the other groups at the 24th and 96th hours [p=0.000]. In this study we found that the most efficient Gln administration technique in sepsis was enteral administration together with parenteral administration due to trophic effect on the intestinal mucosa, decrease of reproduction in tissue and blood cultures, immunomodulator effect, and approximately the same cost as parenteral application
ABSTRACT
To investigate the safety of laparoscopic intervention for diagnosis and treatment at 8 mm Hg pressure in one-hour period on acute peritonitis related intra-abdominal sepsis model. In this study, we included 32 female Wistar-Albino rats, weighing 250 +/- 20 g, and divided them into 4 groups. This study was conducted in Istanbul University Experimental Medical Research Institution [DETAE] laboratory from April to May 2009. Intra-abdominal sepsis was created with intraperitoneal [i.p.] one mL [10[9] CFU/mL] Escherichia coli [E. colt] injection, and pneumoperitoneum was formed with CO insufflation at 8 mm Hg pressure for one hour i.p. The rats were administered with: Group 1 - one mL i.p. isotonic saline; Group 2 - one mL i.p. isotonic saline + pneumoperitoneum; Group 3 - i.p E. coli', and Group 4 - i.p. E.coli + pneumoperitoneum. Data were analyzed using the Statistical Package for Social Sciences version 15 for Windows [SPSS Inc, Chicago, IL, USA]. Fever and leukocyte values were considered high in Groups 3 and 4 compared with Groups 1 and 2 [p=0.001]. The administered reproduction ratio of the E. coli strain was determined as 0% in Groups 1 and 2, and 100% in Groups 3 and 4. In this study, as pneumoperitoneum was formed for one hour at 8 mm Hg pressure, in case of intra-abdominal derived sepsis where emergency intervention is needed, we consider that laparoscopic approaches with low pressure may be used safely for diagnosis and treatment