ABSTRACT
To determine the effect of thymoquinone and methotrexate on lung in pristine induced inflammation in rats. It was a comparative study which was carried out at Post Graduate Medical Institute Lahore. Rats were divided into four equal groups each consisting of 8 rats [n=8]; group A served as healthy control, group B was taken as positive control, group C was treated with thymoquinone and group D with methotrexate. Arthritis developed within two weeks after a single pristane injection which is assessed through total leukocyte count. Total leukocyte count was taken at day 0, 15 and 30 while lung tissue for histopathology was taken at the time of dissection [day 30]. The result of this study indicated that development of arthritis was accompanied by significant raise in total leukocyte count and the presence of inflammatory cells in lung histopathological sections as compared to healthy control groups. Both thymoquinone and methotrexate significantly reduced total leukocyte count and number of inflammatory cells as compared to positive control group of arthritic rats. Evaluation of results supported the beneficial effects of both thymoquinone and Methotrexate in lung inflammation produced by murine model of rheumatoid arthritis. Thus thymoquinone can be a potential therapy for rheumatoid lung and needs further evaluation in human models