ABSTRACT
Transplantation of hematopoietic progenitor cells (HPC) from bone marrow and mobilized peripheral blood is a standard therapy in malignant and non malignant diseases. The lack of suitable donors is an important limitation. The discovery that umbilical cord blood (CB) contains high numbers of HPC that can be used as an alternative source for allogeneic stem cell transplantation led ITMO to establish BANCEL, the first Argentine and Latinoamerican experience of its kind. The blood remaining in the umbilical cord and in the placenta was requested from women who were in the last quarter of pregnancy. An informed consent together with a medical record focused on family disease was completed. Out of 65 donations, 55 (85 per cent) were collected and 51 (78 per cent) were cryopreserved. Mean collected volume was 110 ml with 68 per cent (75 ml) reduction and mean cryopreservation of 35 ml; ABO and Rh blood group systems were determined, HLA, class I, A and B loci, and class II, DR locus were typed by molecular biology methods using PCR-SSOP. Infectious disease screening was carried out for brucellosis, syphilis, Chagas, hepatitis B and C, HIV I and II, HTLV I and II, toxoplasmosis and cytomegalovirus. Two positive units for hepatitis B (anticore) and two positive units for Chagas were discarded. The quantity of total nucleated cells (TNC), CD34+ cells and the clonogenic capacity were determined twice at the collection and after the procedures of volume reduction previous to cryopreservation. A 5 per cent reduction in both TNC and CD34 cells and a 10 per cent in the colony forming units (CFU) were detected. A good correlation coefficient between TNC and CFU was obtained.
Subject(s)
Humans , Male , Female , Pregnancy , Infant, Newborn , Blood Banks , Fetal Blood , Hematopoietic Stem Cells , Cryopreservation , Hematopoietic Stem Cell TransplantationSubject(s)
Humans , Infant, Newborn , Pregnancy , Asphyxia Neonatorum/diagnosis , Consensus Development Conferences as Topic , Cerebral Palsy/diagnosis , Cerebral Palsy/epidemiology , Cerebral Palsy/etiology , Cerebral Palsy/prevention & control , Perinatal Care , Fetal Monitoring , Fetal Mortality , Hypoxia, Brain/complications , Hypoxia, Brain/mortality , Infant MortalityABSTRACT
El embarazo abdominal que llega al término de la gestación, es al mismo tiempo un problema infrecuente y dramático. Una cuidadosa revisión de la literatura mundial sobre casos relatados de embarazo abdominal de término con madre y niño vivos, permite afirmar que ésta es una condición extremadamente rara. Se presenta un caso de embarazo extrauterino avanzado, que finalizó con un recién nacido con peso elevado para la edad gestacional, sano y con un desarrollo posnatal normal. La madre y el niño fueron dados de alta al 7§ día después del parto. Se hacen consideraciones sobre la dificuldad diagnóstica, la asociación entre D.I.U. y embarazo ectópico y los posibles errores cometidos en el diagnóstico