ABSTRACT
To study antihyperlipidemic properties of aqueous extract of Cassia fistula leaves in mice. Experimental study. This study was conducted at Pharmacology Laboratory of Islamic International Medical College, Rawalpindi, and National Institute of Health, Islamabad from February to April 2011. 40 male Balb/c mice were used which were randomly divided into 4 groups i.e. Group A, B, C and D, each group containing 10 mice. Duration of study was 8 weeks after acclimatization [1 week]. Group A served as control group [received standard mice diet], Group B as disease control [received 2% cholesterol diet] and Group C and D as Cassia fistula groups [received 2% cholesterol diet + aqueous extract of Cassia fistula leaves 250 and 500 mg/kg respectively]. At the end of 8 weeks, blood samples of all the mice were analyzed. In disease Group B, there was significant rise in the levels of serum total cholesterol, triglycerides and LDL-C and decline in HDL-C. In Group C and D [Cassia fistula groups], there was much less rise in cholesterol, triglycerides and LDL-C especially in Group D. Also, there was significant increase in HDL-C level in group D. The results demonstrate that administration of aqueous extract of Cassia fistula leaves, along with cholesterol diet, not only prevented the rise in serum cholesterol, triglycerides and LDL-C levels but also increased the HDL-C level, demonstrating antihyperlipidemic properties of the extract
ABSTRACT
Assessment and Comparison of blood group typing among male, female urban and rural population of Tehsil Hassan Abdal, District Attock, Punjab, Pakistan. A total of 400 healthy male, female volunteers belonging to both rural and urban areas were tested for blood group typing. In the selected population in case of ABO blood groups A, B and O were pre dominant. In the same population in case of Rh blood groups Rh + was pre dominant. The assessment and comparison of blood groups in various subclasses of selected population can be beneficial in effective management of blood bank, swift management of disasters requiring massive blood transfusions and development of blood donor society
ABSTRACT
To study hypoglycemic properties ofpowdered plant and aqueous extract of Allium sativum [Garlic] bulbs in type-II diabetics. Experimental human study. This study was conducted at the Hamdard Institute of Pharmaceutical Sciences Islamabad and Army Medical College Rawalpindi from_. The study was performed on 45 humans, which were divided into 3 groups i.e. Group A, B and C. Group A comprises of 15 patients of type-II diabetes, taking no drugs for diabetes. Group B comprises of 15 patients of Type-II diabetes taking oral hypoglycemic agents with inadequate control of blood sugar levels. Group C was control group, containing 15 healthy volunteers. The study was divided into 2 phases. Initially, after baseline sampling for blood glucose and urinary glucose, all the subjects were given powdered bulbs of Allium sativum orally, at low [20 mg/kg/d], intermediate [30 mg/kg/d] and high [45 mg/kg/d] doses, for 14 days. At day 15, blood and urine sampling was done. After 1 week, all the subjects were administered aqueous extract of Allium sativumbulbs orally, at low [20 mg/kg/d], intermediate [30 mg/kg/d] and high [45 mg/kg/d] doses, for 14 days. At the end, sampling was done again. Both dry powdered plant and aqueous extract of bulbs of Allium sativum [Garlic] decrease blood and urine glucose levels in type-II diabetics, especially in the groups who were taking oral hypoglycemics and had inadequate control of blood glucose previously. Allium sativum has significant hypoglycemic activity, particularly in high dose, and can be combined with oral hypoglycemic agents in type-II diabetics
ABSTRACT
DMA analysis for the Genetic Mapping of Candidates of Deafness Genes in Pakistani Families. It was a cross sectional study. Department of Biochemistry/Molecular Biology, Quaid I Azam University, Islamabad Pakistan. The Clinical examination, biochemical tests, interpretation of results and preparation of results completed in approximately one year, 2006 2007. Study was conducted on two Pakistani families. Two Pakistani families labeled as family A and B were selected for the study. Family A comprises of three normal and three affected [Deaf] individuals. Family B comprises of two normal and four affected [Deaf] individuals. The blood samples were immediately dispatched to Molecular genetic laboratory, Quaid I Azam University, Islamabad for analysis 2006 2007. In family A, linkage was established to DFNB47 locus on the chromosome 2p25.1-p24.3. In family B, linkage to DFNB1 locus was excluded first by genotyping polymorphic microsatellite markers linked to the candidate region and then by sequencing GJB2 gene. The genetic mapping of candidates of deafness genes brings greater understanding of molecular basis of deafness and would modify the preventive and curative methods