ABSTRACT
?AlM:To compare the tear functions and the impression cytology scores of the patients with primary open angle glaucoma ( POAG ) , ocular hypertension ( OHT ) and normal subjects with healthy ocular surface both functionally and clinically. ?METHODS: Eleven eyes of 11 patients with POAG (mean age: 62. 7±6. 1y), 12 eyes of 12 patients ( mean age:62. 8±6. 4y ) with OHT and 12 eyes of 12 normal subjects ( mean age: 62. 9±6. 03y) were included to this prospective study. The patients with POAG and OHT had been recently diagnosed with these diseases and none of them had taken anti - glaucoma treatment before. ln addition to conjunctival impression cytology, tear break-up time ( TBUT ) and basal Schirmer’s tests ( BST ) were performed. lmpression cytology specimens of each group were graded and scored in the range of 0-3 according to Nelson’s method. Kruskal- Wallis analysis and Dunn’s multiple comparison tests were used for statistical analysis. ?RESULTS:The mean BST values were 10. 4±1. 3, 10. 9±1. 2 and 11. 1±1. 1 mm/5min of POAG, OHT and control groups respectively. The differences among the BST values of the POAG, OHT and control group were not statistically significant (P=0. 33). The mean TBUT values were 11. 2±1. 1, 11. 3±1. 1 and 11. 8±1. 2s in POAG, OHT and normal subjects respectively. The differences among the BUT values of the POAG, OHT and control group were not statistically significant (P=0. 35). Six eyes (54. 5%) revealed grade 0 and 5 eyes ( 45. 5%) revealed grade 1 impression cytology scores in POAG group. Six eyes (50%) revealed grade 0 and 6 eyes (50%) revealed grade 1 impression cytology scores in OHT group and 6 eyes (50%) revealed grade 0 and 6 eyes (50%) revealed grade 1 impression cytology scores in normal subjects ( P =0. 97). ?CONCLUSlON: Oxidative stress may cause glaucoma, ocular surface diseases, lacrimal gland malfunction and a decrease in mucus secretion ofgoblet cells in all of the body. There were no significant differences between the impression cytology scores of patients with POAG, OHT and normal subjects.
ABSTRACT
Estudos recentes demonstram que a formação de miringoesclerose pode ser reduzida pela aplicação de enzimas e elementos antioxidantes. OBJETIVO: Investigar a eficácia da coenzima Q10 na prevenção de miringoesclerose experimentalmente induzida. MÉTODO: Quarenta e oito ratas Wistar albinas saudáveis sofreram miringotomia e foram divididas aleatoriamente em quatro grupos. O Grupo A não recebeu tratamento algum; o Grupo B recebeu coenzima Q10 por via oral; o Grupo C foi tratado com soro fisiológico tópico; e o Grupo D recebeu coenzima Q10 tópica. No 15º dia de tratamento, as membranas timpânicas foram examinadas por otomicroscopia. As lesões miringoescleróticas foram documentadas de forma semiquantitativa por meio de uma escala de quatro pontos. Após a coleta, as membranas timpânicas foram avaliadas por histopatologia. RESULTADOS: No grupo D (coenzima Q10 tópica) foi observada a ocorrência de otite nos primeiros quatro dias do estudo, o que levou à sua exclusão do estudo. O exame de otomicroscopia não revelou diferenças significativas entre grupos em termos de formação de miringoesclerose (p = 0,241). Diferenças estatisticamente significativas foram observada quando os exames histopatológicos do grupo A foram comparados aos dos grupos B e C (p = 0,004; p < 0,001, respectivamente). Não houve diferença significativa entre os grupos B e C (p = 0,160). CONCLUSÃO: A administração oral de coenzima Q10 não reduziu a formação de miringoesclerose nos ratos submetidos à miringotomia.
Recent studies have shown that the formation of myringosclerosis could be reduced by the application of antioxidant enzymes and elements. OBJECTIVE: The aim of this study was to investigate the effectiveness of coenzyme Q10 on the prevention of experimentally induced myringosclerosis. METHOD: Forty-eight healthy female wistar albino rats were bilaterally myringotomized and divided into four groups randomly. Group A received no treatment, group B was administered oral coenzyme Q10. Group C was treated with topical saline solution, group D received topically coenzyme Q10. On the 15th day of treatment, tympanic membranes were examined by otomicroscopy. Myringosclerotic lesions were documented semiquantitatively by using 4-point scale. After harvesting tympanic membranes were evaluated histopathologically. RESULTS: In group D (topical coenzyme Q10), we observed otitis within the first four days of the study and this group was excluded from the study. Regarding otomicroscopic examinations, there were no significant differences among groups in myringosclerosis formation (p = 0.241). When group A (non treatment) compared to groups B and C regarding histopathologic examination, the results demonstrated statistical significant differences (p = 0.004; p < 0.001), respectively. There was no statisticaly significant difference between groups B and C (p = 0.160). CONCLUSION: Oral administration of coenzyme Q10 did not reduce myringosclerosis formation in myringotomized rats.