ABSTRACT
Chikungunya fever is caused by the chikungunya virus [CHIKV], a mosquito-borne emerging pathogen, which was first revealed on the borders of Mozambique and Tanzania in 1952. Currently it is stretched over 40 countries globally. It is an arthropod-born virus endemic in Africa, Southeast Asia and India. Aedes aegypti and Aedes albopictus are the mosquito vectors which spread this virus. Blood, saliva and urine are the samples for investigation. Since there is no definite treatment available, identifying ways to abolish mosquito populations is the most useful strategy to control the disease. As the virus has facility for global spread, there is need to take preventive measures as well as rapid diagnostic tests to improve identification of Chikungunya patients
ABSTRACT
To complement an earlier analysis of protein alterations in plasma from uremic versus healthy subjects by addition of further LC-MS/MS analysis to the previously used MALDI-TOF mass analyses. Sequence identifications of tryptic peptides from SDS gel electrophoretic fractions of immunodepleted and HPLC-fractionated plasma was performed from seven chronic kidney disease stage 5 patients [age 55 +/- 14 years, glomerular filtration rate 6.9 +/- 2.9 mL/minute/1.73 m[2]] and from seven matched controls. About twice as many proteins were increased in uremic plasma as the previously identified. The identifications included proteins that consistently complement the two identification patterns regarding separate subunits from the same protein complex. Mass spectrometric analysis is applicable to complex plasma proteomes in clinical settings. The LC-MS/MS technique, based on individual peptide sequence analyses, gives increased identifications and also demonstrates feasibility of this technique in clinical practice