ABSTRACT
The study evaluated the antibiotic resistance patterns of Helicobacter pylori strains against metronidazole and clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori strains investigated were resistant to metronidazole, ciprofloxacin, and clarithromycin respectively but all were susceptible to amoxicillin and tetracycline. RdxA truncation was found only in metronidazole-resistant strains. In such strains, reported are eight and two novel mutations in the rdxA and frxA genes respectively. Two-point mutations in the 23S rRNA genes of clarithromycin-resistant strains were detected. A high prevalence of metronidazole resistance was found in Cuban H. pylori strains. Mutations in the rdxA gene may contribute more significantly than frxA gene to the high level of resistance to metronidazole. This study supports the need to continue monitoring the antibiotic susceptibility in H. pylori in Cuba to guide the treatment of such infection.
ABSTRACT
The colorectal neoplasia is the second cause of death from neoplasia in our country, and in international statistics, blaming forthis, the dietetic habits of industrialized countries having a high content of satured fat, cholesterol, refined carbohydrate, red meat, and with few dietetic fibers. In the last years special attention has been focused to the action of the total biliar acids (TBA) primarily the secondary ones, over the colon mucosa, showing evidences of cancerous effects. Recently, American authors have published the favoring action of the cellulose fiber over the TBA through a catalytic reaction and their polysterification, inactivating them in their aggressive action over the colon mucosa. Through these experiences and willing to prove the action of the product, we have treated with mycrocristalline cellulosa (Microcel Lab. Blanver, Brasil) 20 patients of the Institute of Gastroenterology of Havana City, who showed high figures of TBA in stools for 2 months, compared with the 5 g. dose per day. Another group of 20 patients also with high figures of TBA in stools being treated with corn fecula same dose, same time by equal time. making every month determinations to both groups, determinig that in the first group the figures of TBA in stools were normalized in 95 per cent the first month of treatment and in 100 per cent the second month. The second group had only an answer of 65 per cent the first month and of 80 per cent the second month, which shows evidently the high efficacy of Microcel in reducing the TBA in stools.