ABSTRACT
A method for residual determination of 5 pyrethroid pesticides in Anoectochilus roxburghii by cloud point extraction-back extraction-GC-MS was established. PEG 6000 was used as extraction agent and isooctane was used for back-extractant. The con- tent was calculated by external standard method. The linear range was from 15 to 2 000 μg x kg(-1) with the good correlation coefficients (0.955-0.999). The recoveries at spiked concentrations of 50-500 μg x kg(-1) ranged from 85.12% to 101.6%. The limit of detection and quantification of 5 pyrethroid pesticides were in the range of 0.63-3.10 μg x kg(-1) and 2.10-10.31 μg x kg(-1), respectively. The proposed method can be applied to the determination of pyrethroid pesticides residues in A. roxburghii.
Subject(s)
Chemical Fractionation , Methods , Gas Chromatography-Mass Spectrometry , Methods , Orchidaceae , Chemistry , Pesticide Residues , Chemistry , Pyrethrins , ChemistryABSTRACT
Emulsified isoflurane is a novel intravenous anesthetic, which is a lipid emulsion of isoflurane. As some drugs have a QTc-prolongation effect which can increase a risk of arrhythmia, this study was to evaluate the effects of emulsified isoflurane on the QTc interval
This was a single-center, randomized, single-blind, non-comparative study. Subjects were randomly allocated to receive an intravenous bolus injection of 22.63, 38.26, or 49.73 mg/kg emulsified isoflurane, respectively
Standard 12-lead electrocardiograms were recorded before administration and at 28 timepoints after administration
Blood samples and the end-tidal isoflurane concentrations were collected for pharmacokinetic analysis. The primary target variable was the QTcF change from baseline at each time point. A two-sided 90% confidence interval [CI] was calculated for a QTcF change from baseline at each timepoint. The maximal 90% CIs of the mean QTcF from the baseline for 22.63, 38.26 and 49.73mg/kg emulsified isoflurane were 2.52-21.18 ms, 15.66-35.90 ms, and 17.65-40.71 ms, respectively
Non-significant relationship was observed between QTcF and the plasma concentration [or the end-tidal isoflurane concentration]
Single intravenous dose of emulsified isoflurane of the anticipated therapeutic dose or supra-therapeutic doses was associated with a potential dose-dependent and non-concentration-related QTc-prolongation effect
ABSTRACT
Objective To study the role of insulin-like growth factor 2 (IGF- Ⅱ ) in the proliferation and degeneration of hemangiomas. Methods The expression of IGF- Ⅱ and Ki-67 was detected with SP im-munohistochemical method in 52 specimens of hemangiomas, 27 of vascular malformation and 5 of normal skin. The expression of IGF-Ⅱ mRNA was examined by reverse transcription polymerase chain reaction (RT-PCR) in specimens from 9 cases of hemangiomas and 5 of vascular malformation. Results The labelling indexes (LI) of IGF- Ⅱ and Ki-67 and the expression of IGF- Ⅱ mRNA were significantly higher in heman gioma than those in vascular malformation and normal skin (P