ABSTRACT
In our program to search for new AMP-activated protein kinase (AMPK) activators from plants that exert potential anticancer property, we found that an EtOAc extract of Myristica fragrans (nutmeg) activated AMPK enzyme in human breast cancer MCF-7 cells. Two major diarylbutane-type lignans, macelignan and meso-dihydroguaiaretic acid (MDGA), were isolated as active principles from this extract. Treatment of breast cancer cells with two compounds induced cellular apoptosis, evidenced by cleavage of poly-(ADP-ribose) polymerase (PARP) and Ser 15 phosphorylation of p53. Moreover, macelignan and MDGA significantly inhibited the colony formation of MCF-7 breast cancer cells on soft agar. Intraperitoneal injection of macelignan and MDGA (20 mg/kg) suppressed the tumor growth of 4T1 mammary cancer cells. These results indicate that the chemopreventive effects of two major diarylbutane-type lignans from Myristica fragrans (nutmeg) may be associated with induction of apoptosis presumably through AMPK activation.
Subject(s)
Humans , Agar , AMP-Activated Protein Kinases , Apoptosis , Breast Neoplasms , Breast , Injections, Intraperitoneal , Lignans , MCF-7 Cells , Myristica , PhosphorylationABSTRACT
61 patients (age 20-59 years) treating in the Department for Internal Medicine were investigated using descriptive method. These patients have not any endocrine disease. There are significant difference in serum lipid and lipoprotein levels between patients with cerebrovascular stroke and general population. Common types of dyslipidemia are that can cause risk for developing arteriosclerosis, in which type IV is 59.02%.