ABSTRACT
More than 20 percent of the world's biodiversity is located in Brazilian forests and only a few plant extracts have been evaluated for potential antibacterial activity. In the present study, 705 organic and aqueous extracts of plants obtained from different Amazon Rain Forest and Atlantic Forest plants were screened for antibacterial activity at 100 µg/ml, using a microdilution broth assay against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli. One extract, VO581, was active against S. aureus (minimum inhibitory concentration (MIC) = 140 µg/ml and minimal bactericidal concentration (MBC) = 160 µg/ml, organic extract obtained from stems) and two extracts were active against E. faecalis, SM053 (MIC = 80 µg/ml and MBC = 90 µg/ml, organic extract obtained from aerial parts), and MY841 (MIC = 30 µg/ml and MBC = 50 µg/ml, organic extract obtained from stems). The most active fractions are being fractionated to identify their active substances. Higher concentrations of other extracts are currently being evaluated against the same microorganisms.
Subject(s)
Anti-Bacterial Agents/pharmacology , Gram-Negative Bacteria , Gram-Positive Bacteria , Plants, Medicinal , Anti-Bacterial Agents/isolation & purification , Brazil , Enterococcus faecalis , Escherichia coli , Microbial Sensitivity Tests , Plant Extracts , Pseudomonas aeruginosa , Staphylococcus aureus , TreesABSTRACT
O extrato orgânico obtido do caule de Tovomita sp. apresentou atividade antibacteriana significativa contra Staphylococcus aureus (CIM = 460 ?µg/mL e CBM = 490 µg/mL), Enterococcus faecalis (CIM = 500 ?µg/mL e CBM = 540 µg/mL) e Pseudomonas aeruginosa (CIM = 300 ?µg/mL e CBM = 400 µg/mL). As frações obtidas do extrato orgânico apresentaram atividade antimicrobiana contra E. faecalis (CIM F1 = 570 µg/mL e CBM F1 = 840 µg/mL; CIM F2 = 480 µg/mL e CBM F2 = 720 µg/mL, respectivamente) e contra P. aeruginosa (CIM F1 = 310 µg/mL e CBM F1 = 570 µg/mL; CIM F2 = 310 µg/mL e CBM F2 = 460 µg/mL, respectivamente).
The organic extract obtained of the stem of Tovomita sp. did it present significant antibacterial activity against Staphylococcus aureus (CIM = 460 µg/mL and CBM = 490 µg/mL), Enterococcus faecalis (CIM = 500 µg/mL and CBM = 540 µg/mL) and Pseudomonas aeruginosa (CIM = 300 µg/mL and CBM = 400 µg/mL). The obtained fractions of the organic extract present antibacterial activity against E. faecalis (CIM F1 = 570 µg/mL and CBM F1 = 840 µg/mL; CIM F2 = 480 µg/mL and CBM F2 = 720 µg/mL, respectively) and against P. aeruginosa (CIM F1 = 310 µg/mL and CBM F1 = 570 µg/mL; CIM F2 = 310 µg/mL and CBM F2 = 460 µg/mL, respectively).