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1.
Braz. dent. j ; 13(3): 162-165, 2002. ilus, graf
Article in English | LILACS, BBO | ID: lil-338535

ABSTRACT

The purpose of the present study was to evaluate the influence of short course topical application of carbamide peroxide on proliferating cell nuclear antigen (PCNA) immunohistochemical expression in the oral tongue mucosa of rats. Twelve male Wistar rats were submitted to topical application of 10 percent carbamide peroxide on one side of the dorsal tongue once a week for three consecutive weeks. Only distilled water was applied on the control side. The animals were killed on days 0, 10, and 20 after the last application. The tongue was fixed in buffered formalin for 24 h and embedded in paraffin. Tissue blocks (3 æm) were subjected to the biotin-streptavidin amplified system for identification of PCNA. The percentage of epithelial-positive basal cells in each side of the tongue mucosa was calculated. The results demonstrated that topical application of 10 percent carbamide peroxide increases PCNA immunohistochemical expression on the basal layer of the oral mucosa epithelium of rats on day 0 after treatment. In conclusion, short-course use of carbamide peroxide induces transient epithelial cell proliferation of the oral mucosa of rats


Subject(s)
Animals , Male , Rats , Proliferating Cell Nuclear Antigen/biosynthesis , Epithelial Cells , Mouth Mucosa , Peroxides/toxicity , Tooth Bleaching , Cell Division , Tongue/cytology , Rats, Wistar
2.
Arq. odontol ; 36(1/2): 91-100, 2000. ilus, tab
Article in Portuguese | LILACS, BBO | ID: lil-500975

ABSTRACT

Esse estudo visa avaliar a microinfiltração em coroas totais, cimentadas por 2 técnicas diferentes (aplicação do agente em toda a superfície interna ou apenas nas paredes laterais internas das coroas) sendo utilizado como agente cimentante cimento resinoso associado ou não a um sistema adesivo. Trinta e dois molares humanos extraídos e hidratados foram preparados para a coroa total. As coroas foram assentadas com a pressão digital, levadas à prensa para serem carregadas. Foram impermeabilizadas, imersas em solução de azul de metileno, incluídas em resina acrílica e cortadas longitudinalmente . Os resultados deste estudo indicam que o GRUPO 1 (aplicação do cimento em toda superfície interna sem aplicação de adesivo) exibiu maior infiltração. Concluiu-se que não houve diferença significante na microinfiltração entre os grupos, embora diferença significante (a<0.05) possa ser observada quando o grupo 1 foi comparado com os outros grupos separadamente.


Subject(s)
Humans , Male , Female , Dental Cements/adverse effects , Denture, Partial, Fixed, Resin-Bonded/trends , Cementation , Molar/abnormalities
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