ABSTRACT
Abstract The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) and Super EBA(r). A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p<0.05), but there was a similar behavior in lower dilutions (p>0.05). Cell viability of MTA Angelus(r) was superior for 2-day setting (p<0.05), compared with the other two root-end fillings. There were no statistically significant differences between 7-day set MTA Angelus(r) and EndoSequence Root Repair Material Putty(r). Super EBA(r) showed the lowest percentage of cell viability at higher dilutions (p<0.05). Therefore, MTA Angelus(r) and EndoSequence Root Repair Material Putty(r) were less cytotoxic in the highest dilution (1:1) compared with Super EBA(r).
Resumo O objetivo deste trabalho foi avaliar in vitro a citotoxicidade em fibroblastos do ligamento periodontal humano de três cimentos de retrobturação: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) e Super EBA(r). Uma cultura de fibroblastos primários do ligamento periodontal humano foi obtida anteriormente a fim de avaliar a citotoxicidade dos três extratos dos cimentos de retrobturação após 2 e 7 dias de endurecimento. As diluições em série destes extratos (1:1, 1:2, 1:4 e 1:8) foram avaliados em 1, 3 e 7 dias empregando o ensaio colorimétrico metil-tiazol-tetrazólio (MTT). A viabilidade celular foi calculada em base da porcentagem do grupo de controle negativo, que representou 100% de viabilidade de células. As análises estatísticas foram realizadas com o teste t, ANOVA e teste de Kruskal-Wallis a um nível de significância de 5%. Verificou-se que a principal diferença entre os cimentos de retrobturação estava nas diluições mais elevadas (p<0,05) e houve um comportamento semelhante nas diluições mais baixas (p>0,05). A viabilidade celular dos fibroblastos do ligamento periodontal humano foi superior para MTA Angelus(r) de 2 dias de endurecimento (p<0,05), em comparação com os outros materiais de retrobturação. Não houve diferença significante entre MTA Angelus(r) e EndoSequence Root Repair Material Putty(r) de 7 dias de endurecimento. Super EBA(r) mostrou a menor percentagem da viabilidade celular nas diluições mais altas (p<0,05). Portanto, os cimentos de retrobturação MTA Angelus(r) e EndoSequence Root Repair Material Putty(r) foram menos citotóxicos na diluição mais alta (1:1) em comparação com Super EBA(r).
Subject(s)
Humans , Periodontal Ligament/pathology , Root Canal Filling Materials , Cells, Cultured , In Vitro TechniquesABSTRACT
Evaluar ôin vitroõ la resistencia a la penetración bacteriana utilizando dos diferentes técnicas de obturación, compactación vertical y lateral con dos selladores endodónticos, uno a base de polidimetilsiloxano y el otro a base de hidróxido de calcio y resina epóxica frente a una cepa de Enterococcus faecalis ATCC 29212. Materiales y Métodos: Ochenta y cuatro dientes uniradiculares se dividieron de manera aleatoria en 4 grupos (n=20) y dos grupos de control (n= 4). El grupo I se obturó con la técnica de compactación lateral y sellador Sealer 26, el grupo II se obturó con la técnica de compactación vertical y sellador Sealer 26, el grupo III se obturó con la técnica de compactación lateral y sellador Roeko Seal y el grupo IV se obturó con la técnica de compactación vertical y sellador Roeko Seal. Se utilizó Enterococcus faecalis como marcador bacteriano y la penetración bacteriana se evaluó cada hora durante las primeras 96 horas, posterior a esto cada 12 horas durante 15 días. Resultados: Se utilizó la prueba de Kruskal Wallis (p=0.696) para comparar los 4 grupos y la prueba de la U de Mann Whitney para comparar pares de grupos, al comparar las técnicas de compactación según el sellador endodóntico no se encontró diferencias estadísticamente significativas en ambos materiales (Grupo I y II p=1.000, Grupo III y IV p=0.296). Al comparar los selladores endodónticos según las técnicas de compactación no se encontró diferencias estadísticamente significativas (Grupo I y III p=0.328, Grupo II y IV p=1.00). Sin embargo se registró un tiempo máximo de 115 horas para el grupo III y un tiempo mínimo de 18:21 horas para el grupo I. Conclusiones: No se encontró diferencias estadísticamente significativas en cuanto a la resistencia a la penetración bacteriana entre los selladores Roeko Seal y Sealer 26 en combinación con las técnicas de compactación lateral y vertical frente a una cepa de Enterococcus faecalis...
Objectives: The purpose of the study was to assess ôin vitroõ bacterial penetration resistance using two different obturation techniques, vertical compaction and lateral compaction with two endodontic sealers, one based on polydimethylsiloxane and the other based on calcium hydroxide and epoxy resin against Enterococcus faecalis strain ATCC 29212. Methods: Eighty-four uniradicular teeth were randomly divided into 4 groups (n = 20) and two control groups (n = 4). Group I was obturated with lateral compaction technique and Sealer 26, group II with vertical compaction technique and Sealer 26, group III with lateral compaction technique and Roeko Seal and group IV with vertical compaction technique and Roeko Seal. Enterococcus faecalis was used as a bacterial marker and bacterial penetration was assessed monitoring the specimens every hour for the first 96 hours and after this every 12 hours over a period of 15 days. Results: Kruskal Wallis test (p = 0.696) was used to compare the four test groups and the Mann Whitney U test was used to compare pairs of groups. Nevertheless, no statistically significant differences were found comparing compaction techniques as endodontic sealers (Group I and II p=1.000, group III and IV p=0.296). No statistically significant differences were found when comparing endodontic sealers as compaction techniques (Group I and III p=0.328, group II and IV p=1.000). Although there was a maximum of 115 hours for group III and a minimum time of 18:21 hours for group I. Conclusions: No significant differences were found regarding bacterial penetration resistance between Roeko Seal and Sealer 26 in combination with lateral and vertical compaction techniques against a strain of Enterococcus faecalis...
Subject(s)
Humans , Enterococcus faecalis , Bacterial Infections , Dental Restoration, Permanent , Pit and Fissure SealantsABSTRACT
Conociendo que el barrillo dentinario se forma al estar en contacto la pared de dentina con una lima manual o rotatoria es importante recordar que su remoción tiene un propósito doble al tener un componente orgánico e inorgánico para lo cual se recomienda el uso alternado de 10ml de ácido etilendiaminotetracético al 17 % y 10ml de hipoclorito de sodio al 5,25% por 1 minuto, sin embargo la eficacia en la remoción de barrillo dentinario en el tercio apical ha sido menor por tal motivo el objetivo de esta revisión fue recopilar información para dar a conocer el efecto que ha mostrado el uso del ultrasonido en la remoción del barrillo dentinario ya que es inevitable su formación cuando un conducto radicular es instrumentado.
Knowing that the smear layer is formed by contact of dentin wall with a hand file or rotary is important to remember that their removal has a twofold purpose to have an organic and inorganic which is recommended for alternate use 10ml of 17%ethylenediaminetetraacetic acid and 10 ml of 5.25% sodium hypochlorite for 1 minute, but the effectiveness in removing smear layer in the apical third was less for that reason the aim of this review was to gather information to make known the effect it has shown the use of ultrasound in removing the smear layer and its formation is inevitable when a root canal is instrumented.
Subject(s)
Humans , Smear Layer , Air Particle Removal , UltrasonicsABSTRACT
El tratamiento de los dientes inmaduros con pulpa no vital tradicionalmente se realiza a través de una apexificación con hidróxido de calcio, este procedimiento ha estado vigente por muchas décadas en el cual se logra formar una barrera de tejido mineralizado en un periodo de tiempo largo. En la actualidad existen otros métodos que permiten obtener una barrera dura contra la cual se puede realizar la obturación endodóntica, incluso en una sola cita, esto es la formación de un tapón apical mediante el uso de agregado de trióxido mineral (MTA), pero que a la vez no permitirá que continúe el desarrollo del largo radicular ni el engrosamiento de las paredes dentinarias. La revascularización es una nueva opción de tratamiento para estos dientes no vitales que no han concluido su formación radicular; este método consiste en la desinfección del sistema de conductos radiculares, para posteriormente introducir una matriz natural formada por el coágulo sanguíneo, el cual va a proporcionar nuevas células que pueden crecer y producir el cierre del tercio apical, logrando la formación fisiológica del contenido radicular. En el presente reporte de caso presentamos dos piezas dentales tratadas por este método, los cuales han logrado resultados favorables en cuanto al desarrollo radicular (largo y engrosamiento de las paredes).
The treatment of inmature teeth with nonvital pulp is a traditionally performed through a apexification with calcium hydroxide. This procedure has been around for many decades, which does form a mineralized tissue barrier in a period of time. At the present time there are other methods to obtain a hard barrier to be able to perform endodontic filling, even in one appointment this is the formation of an apical cap using mineral trioxide aggregate (MTA), although it does now allow to continue the development of root length and the thickened of the dentine walls Revascularization is a new treatment option for these non-vital teeth that have not completed their root development, this method consist in disinfecting the root canal system, and then inserting a natural matrix formed by the blood clot, which will provide new cells that can grow and produce the apical end, archieving the physiological formation of the root canal. In this case report we present two teeth treated by this method, which have achieved favorable results in terms of root development (length and thickness of the walls).