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1.
Southeast Asian J Trop Med Public Health ; 2002 ; 33 Suppl 2(): 48-56
Article in English | IMSEAR | ID: sea-33976

ABSTRACT

The Philippine Council for Quality Assurance In Clinical Laboratories has conducted two National External Quality Assessment Schemes (NEQAS) in Hematology. The first survey was conducted in December 1999 and the second in August 2000, with 95 and 187 laboratories, using mostly automated analyzers, participating respectively. Control materials were distributed during a two-week period by human network, and analyzed over a six to eight week period. For the first survey, only 36 laboratories (38.0%) submitted results. Data was divided into 4 peer groups based on the manufacturer. Since most of the samples were hemolysed upon analysis, only WBC and HGB parameters were evaluated. No outliers were detected in each peer group after analysis by the 'Peer Group Mean and SDI' method. Using the clinical laboratory improvement act of 1988 proficiency testing criteria (CLIA'88), only 5 results (13.9%) were unsatisfactory for WBC, and all results were satisfactory for HGB. For the second survey, 87 laboratories (47%) responded. Data was divided into 5 peer groups. There were few incidents of sample deterioration. Although majority of the coefficient of variations were acceptable, about 23 (12.6%) participants showed abnormality in at least one parameter after analysis by the 'Peer Group Mean and SDI'. Using CLIA'88, 5 WBC (6.5%), 6 RBC (7.6%), 8 HGB (9.7%), 15 HCT (19.0%), and 7 PLT (8.0%) results were unsatisfactory. In summary, the first NEQAS study served as a pilot study. Valuable lessons were learned for the improvement of the second NEQAS. The second NEQAS study was marked by a much larger sample size and better results.


Subject(s)
Hematologic Tests/standards , Humans , Laboratories/standards , Philippines , Quality Assurance, Health Care
2.
Arq. bras. med. vet. zootec ; 54(1): 42-51, fev. 2002. tab
Article in English | LILACS | ID: lil-324255

ABSTRACT

Corn (CR) and soybean (SR) culture wastes were submitted to extraction with organic solvents for lignin isolation (LGS). The obtained lignin was chemically characterized, and based on studies of functional groups and microanalyses, it was possible to determine the minimum formula. LGS of CR has characteristics that resemble wood and of bamboo lignin, possessing a larger amount of methoxyl groups and vanillin


Subject(s)
Agriculture , Lignin , Glycine max , Waste Products , Zea mays
3.
Arq. bras. med. vet. zootec ; 54(1): 52-56, fev. 2002. tab
Article in Portuguese | LILACS | ID: lil-324256

ABSTRACT

Este experimento foi conduzido com o objetivo de comparar a lignina isolada da palha de milho (LPM-RM) com indicadores utilizados em ensaios de consumo e digestibilidade. Utilizaram-se além da LPM-RM, óxido crômico (Cr2O3), cloreto de itérbio (YbCl3.6H2O), fibra detergente ácido indigestível (FDA - ind), teor de metoxila da lignina (OCH3), lignina Klason (LK) e lignina determinada por espectroscopia no infravermelho (LIG-IV). O coeficiente de digestibilidade diferiu apenas entre a metoxila e os demais indicadores. Todos os indicadores foram semelhantes quanto à recuperaçäo fecal, exceto a metoxila que teve baixa recuperaçäo (44,6 por cento). Os dados de digestibilidade obtidos com LIG-IV, Cr2O3 e YbCl3.6H2O foram semelhantes entre si mas diferentes dos demais indicadores (21 por cento superior no caso do Cr2O3 e 24 por cento inferiores no caso da LIG-IV e do itérbio), quando comparados com a coleta total


Subject(s)
Lignin , Ruminants , Zea mays
4.
Arq. bras. med. vet. zootec ; 51(1): 89-96, fev. 1999. ilus
Article in Portuguese | LILACS | ID: lil-257149

ABSTRACT

O trabalho teve por objetico isolar e caracterizar microscopicamente as ligninas de resíduos agrícolas provenientes das culturas de soja (RS) e de milho (RM), bem como verificar o efeito das ligninas sobre a degradaçäo dos tecidos dos resíduos RM e RS. Por meio da microscopia eletrônica de varredura, estudou-se a ultra-estrutura das ligninas e dos tecidos vegetais de RM e RS. Verificou-se que as ligninas da palha moída do RM e da RS säo morfologicamente diferentes (ultra-estruturas diferentes) e que o desaparecimento dos tecidos digestíveis também é diferente, sendo mais prejudicado no RM


Subject(s)
Animals , Digestion , Lignin/analysis , Microscopy, Electron, Scanning
5.
Arq. bras. med. vet. zootec ; 50(6): 727-30, dez. 1998. ilus, tab
Article in Portuguese | LILACS | ID: lil-265552

ABSTRACT

Este trabalho teve por objetivo isolar e caracterizar ligninas de resíduos agrícolas das culturas de soja (Glycine max L.) (RS) e de milho (Zea mays) (RM). Para tanto, isolou-se a lignina da palha moída (LPM), após moagem em moinho de bolas, seguida de extraçäo por solventes orgânicos. As fraçöes assim obtidas foram analisadas por espectroscopia no infravermelho (IV). Nas fraçöes correspondentes às LPM de soja e milho pôde-se verificar absorçäo em 137Ocm elevado a -1 para RS e em 136Ocm elevado -1 para RM. Esses dados säo característicos de grupos siringila que apresentam absorçäo em torno de 137Ocm elevado a -1. Observou-se ainda em cada lignina a banda em 1265cm elevado a -1, indicativa da presença do grupo guaiacila. Finalmente, uma banda observada em 122Ocm elevada a -1 para RS e 1215cm elevado a -1 para RM pode caracterizar grupamento p-hidroxifenila


Subject(s)
Lignin , Waste Products , Zea mays
6.
Braz. j. med. biol. res ; 31(8): 1095-8, Aug. 1998. tab, graf
Article in English | LILACS | ID: lil-216830

ABSTRACT

We examined the radioprotective effect of aminothiol 2-N-propylamine-cyclo-hexanethiol (20-PRA) on a human leukemic cell line (K562) following various radiation doses (5, 7.5 and 20 Gy) using a source of (60)Co gamma-rays. At 5 Gy and 1 nM 20-PRA, a substantial protective effect (58 per cent) was seen 24 h after irradiation, followed by a decrease at 48 h (11 per cent). At the high radiation dose (20 Gy) a low protective effect was also seen (35 per cent). In addition, the antitumorigenic potential of 10 nM 20-PRA was shown by the inhibition of crown gall formation induced by Agrobacterium tumefaciens. The radioprotective potency of 20-PRA is 10(5)-10(6) times higher than that of the aminothiol WR-1065 N-(2-mercaptoethyl) - 1,3-diaminopropane) whose protective effect is in the 0.1 to 1.0 mM range.


Subject(s)
Humans , Animals , Mercaptoethylamines/pharmacology , Radiation-Protective Agents/pharmacology , Radiation-Protective Agents/isolation & purification , Tumor Cells, Cultured/drug effects
7.
Braz. j. med. biol. res ; 31(7): 901-10, jul. 1998. ilus
Article in English | LILACS | ID: lil-212875

ABSTRACT

Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu(1)-Thr(383) region, identical in LK and HK, contains bradykinin (BK) moieties Arg(363)-Arg(371). LK, HK and their kinin products Lys-BK and BK are involved in several biological processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams'trait), a codon mutation (Arg(178) r stop) has been blamed for the absence of LK and HK. However, using Western blots with 2 monoclonal anti-HK antibodies, one that recognizes the region common to LK and HK and the other that recognizes only HK, I detected ~110-kDa bands in the plasma of this LK/HK-deficient patient vs ~120-kDa bands in normal human and ape plasmas. With polyclonal anti-Lys-BK antibody, which strongly detects BK eleaved at its COOH-terminus in purified HK, I detected ~110-kDa bands in the normal and the deficient plasmas. Western blots with a monoclonal anti-prekallikrein (PK) antibody showed that surface activation of PK and distribution of PK activation products, both dependent on HK, were similar in these plasmas. These findings suggest that a mutant gene yielded a kininogen-like species possibly involving aberrant mRNA splicing - structurally different from normal HK, but apparently with the capacity to carry out seemingly vital HK functions.


Subject(s)
Female , Humans , Blood Coagulation Disorders/genetics , Kininogen, High-Molecular-Weight/genetics , Kininogen, Low-Molecular-Weight/genetics , Kininogens/genetics , Plasma/chemistry , Antibodies, Monoclonal/isolation & purification , Blood Coagulation Disorders/immunology , Blotting, Western , Kininogen, High-Molecular-Weight/immunology , Kininogen, Low-Molecular-Weight/immunology , Kinins/isolation & purification , Mutation , RNA, Messenger/genetics
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